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PROFESOR: JAVIER JACK CALDERÓN

NOMBRE: SOFIA MATHEWS BARRERA

 TEMA: FACTORES QUE AFECTAN LAACTIVIDAD ENZIMATICA

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FACTORES QUE AFECTAN LA

ACTIVIDAD ENZIMATICA

1. OBJETIVOS• Veri!"r #i $" !%&!e&'r"!i(& i&)*+e e& $" e!ie&!i"

,e $" !"'-$i#i# ,e $" e&i/"• Veri!"r #i $" 'e/0er"'*r" i&)*+e e& $" e!ie&!i" ,e

$" !"'-$i#i# ,e $" e&i/"

• Veri!"r #i e$ PH i&)*+e e& $" e!ie&!i" ,e $"

!"'-$i#i# ,e $" e&i/". MATERIALES

• A/i$"#" S"$i2"$• A3*" ,e#'i$","• A$/i,(&• HCL 145• NAOH 145

6. PROCEDIMIENTO

I. VR 7 VS

1814 184 1894 184 181;41MLAMILASA<=ML DEHO

DESTILADA

1ML 1814<=ML HODESTILADA

1ML184<=ML HODESTILADA

1ML 1894<=ML DEHODESTILAD

A

1ML 18<=ML DEHODESTILA

A

1814 184 1894 184 181;41ML 1814<9ML

1ML 184<9ML

1ML 1894<9ML

1ML 184<9ML

1ML 181<9ML

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ALMIDÓN ALMIDÓN ALMIDÓN ALMIDÓN ALMIDÓ

II. VR 7 VS >T?@

1 61ML AMILASA<BM HIRVIENTE <9ML ALMIDÓN<BM 6?C 7 1

1ML AMILASA<9ML ALMIDON<BM 6?C 7 1

1ML AMILASA<BAO DE HIELO <9ML ALMIDÓN<BM DE HIELO

III. VR 7 VS >PH@

1 61ML AMILASA< TS HCL 145<

9ML ALMIDÓN<BM 6? C 7 1

1ML AMILASA<9ML ALMIDÓN<

BM 6?C 7 1

1ML AMILASA< TS NAOH 145<

9ML ALMIDÓN<BM 6?C 7 1

9. RESULTADOS

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I. VR7VS

II. VR7VS>T?@

III. VR7VS>PH@

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. DISCUSIÓNE& e$ 3r"!% *&% 0%,e/%# 2i#*"$i"r *e !%&G%r/e ""$" !%&!e&'r"!i(& $" e!ie&!i" e# /-# "" e& e$ *e,e'er/i&"/%# !%& e$ $*3%$ $*e3% ,e 14 /i&*'%# *e $"

"/i$"#" e& "*e$ 0ri/er '*% 1814 %'ie&e *& !%$%r!"r"/e$% /%#'r"&,% *&" r-0i," e!ie&!i" ,e !"'-$i#i# 0%r *e /*e#'r" *&" i,r%$i#i# '%'"$. Si& e/"r3% e& e$'*% 184 0%,e/%# %#er2"r *& !%$%r 2i%$-!e%/%#'r"&,% *&" i,r%$i#i# 0"r!i"$ + 0%r $'i/% e& $%#$'i/%# 're# '*%# #e $%3r( %#er2"r *& !%$%r "*$i&'e% *&" i,r($i#i# &*$". P%r $% *e 0"r" $" 0r-!'i!",ee/%# %'e&er $" i,r($i#i# !%/0$e'" ,e '%,%# $%#

'*%# 0%r $% *e 0%,e/%# ,e!ir *e e$ '*% 1814 e# /-#e!ie&'e " $%# 14 /i&*'%# e$ 184 e# e!ie&'e " $%# 64/i&*'%# e$ 1894 &" $%# 4 /i&*'%# e$ 184 " $%# 4/i&*'%# + e$ 181;4 " $%# =4 /i&*'%#.A e#%# 'ie/0%# ,e'er/i&",%# 0%,e/%# !%/0r%"r $"!"'-$i#i# e& $" e&i/" ",e/-# ,e $" e!ie&!i" 0*e# !%/%"&'e# /e&!i%&",% !%&G%r/e $" "" !%&!e&'r"!i(& ,e"/i$"#" $" e!ie&!i" e# /-# $e&'".

E&'%&!e# 0%,e/%# "r/"r *e $" 2e$%!i,", ,e $"re"!!i(& e# ,ire!'"/e&'e 0r%0%r!i%&"$ " $" !"&'i,", ,ee&i/"# ,i#0%&i$e#. Si& e/"r3% e#'% &% *iere ,e!ir*e *& "*/e&'% !%'"&'e e& $" !%&!e&'r"!i(& ,e $"#e&i/"# ,"r- $*3"r " *& "*/e&'% !%'"&'e ,e $"2e$%!i,", ,e re"!!i(&. M-# ie& *&" /*+ "$'"

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!%&!e&'r"!i(& ,e e&i/"# e& ,%&,e '%,"# $"# /%$!*$"#,e #*#'r"'% +" #e *'i$i"& &% 'ie&e &i&3& i/0"!'% #%re$" 2e$%!i,", ,e re"!!i(& %#e" "r- /"+%r e!ie&!i".

E& e$ 3r"!% ,%# 0%,e/%# %#er2"r $" 'e/0er"'*r" e&'re# '*%# e& e$ 0ri/er% #e ,e#&"'*r"$i" ,ei,% " *e $""/i$"#" e#'- e0*e#'" " *&" 'e/0er"'*r" e$e2"," +"*e "+ *& !%*e e&'re /%$!*$"# e& $" "/i$"#" !%/%!%e!*e&!i" ,e$ "*/e&'% ,e $" e&er3" !i&'i!"0r%,*!ie&,% $" r*0'*r" ,e $"# *&i%&e# ,i$e# "!ie&,%*e 0ier," #* 0r%0ie,", !"'"$'i!". C%/% $" 'e/0er"'*r"e$e2"," r%/0e $" "/i$"#" e#'e '*% #e '%r&" ,e !%$%r

"*$ /"ri&% e#'e '*% 0re#e&'" *&" i,r%$i#i# &*$" +"*e e$ #*#'r"'% *e," #%$% 0%r $% *e &% "+!"'"$i",%r.E& e$ '*% ,%# $" "/i$"#" "!'" e!ie&'e/e&'e/%#'r"&,% *& !%$%r !"r"/e$% 'r"#$!i,% ,%&,e $"'e/0er"'*r" i&)*+e e& e$ re#*$'",% 0r%2%!"&,% *&"i,r($i#i# !%/0$e'".E& e$ '*% 're# $" "/i$"#" "$ e#'"r e& *& "% ,e ie$% &%#e ,e#&"'*r"$i" #i &% 2" " i&iir 0*e# e$ Gr% 2" " "!'*"r

0"r!i"$/e&'e !%/% i&ii,%r +" *e " /"+%r Gr% /"+%ri&ii!i(& e&i/-'i!". E#'e '*% 0re#e&'" *&" i,r%$i#i#0"r!i"$.

P%r $'i/% e& e$ 3r"!% 're# 0%,e/%# %#er2"r e$ 0 ,e$%# 6 '*%#. E& e$ '*% *&%/*e'#r" 0 ,e 1 0%,e/%#,e!ir *e e#'" e& *& /e,i% -!i,% 0%r $% *e #e e#'-,e#&"'*r"$i"&,% 0er,ie&,% #* 0r%0ie,", !"'"$'i!".

E& e$ '*% "$!"&" *& PH *e e# !%i,er",% *& 0%0'i/% 0*e# e#'" e& e$ r"&3% ,e *& 0 &e*'r%.  e& e$ '*% 6 %'ie&e *& 0 19 e#'e '*% #e e&!*e&'r"e& *& /e,i% "$!"$i&% 0%r $% *e '"/i& #e !%i,er",e#&"'*r"$i",% + e# i&!%$%r% 0%r*e e$ /e,i% "$!"$i&%i/0i,e *e #e 'i" ,e !%$%r "*$ "$ "3re3"r e$ $*3%$.

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C"e re#"$'"r *e $%# *e %'*2ier%& e$ 0 1 + 19 &%e#'-& ,e#&"'*r"$i-&,%#e #%$% *e " /e,i," *e #e"$e"& ,e$ r"&3% &e*'r% #e $e !%i,er" !%/% *&",e#&"'*r"$i"!i(&.

;. CONCLUSION• P%,e/%# !%&!$*ir *e e$ 0 ,e $" "/i$"#" #e

e&!*e&'r" e&'re *& r"&3% ,e ; .• L" "/i$"#" 'ie&e *&" r-0i," e!ie&!i" !*"&,% e#'-

e& *&" !%&!e&'r"!i(& /"+%r.• C*"&,% $" "/i$"#" #e ,e#&"'*r"$i" 0%r *& G"!'%r ,e

'e/0er"'*r" 0ier,e #* "!'i2i,", !"'"$'i!".

• A /"+%r !%&!e&'r"!i(& /"+%r e# $" e!ie&!i"!"'"$'i!".