Grup de Sensors & Biosensors GSB · HRP-antiDig mismatch DNA digoxigenin probe magnet magnetic...
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Transcript of Grup de Sensors & Biosensors GSB · HRP-antiDig mismatch DNA digoxigenin probe magnet magnetic...
GSB[Fundación (1983)]Permanentes (6) Salvador Alegret, CU
Jordi Bartrolí, CUJulian Alonso, CUEsteve Martínez, TUManel del Valle, TUFrancisco Céspedes, AU
Contratados (4) Dmitri Muraviev, RyCMa. José Esplandiu, RyCMa. Isabel Pividori, LUMireia Baeza, LUArben Merkoçi*, ICN
Postdocs (1)Postgrados > 25
GSBtaxonomía evolutiva
1983 1993 2003Potentiometricelectrodes/FIA 1988
GSB
Convergingtechnologies
Biosensor
BioSens
IntegratedAnalyticalSystems
ABIArtificial
BiosensingInterfaces
Sensors
GRUP de SENSORS i BIOSENSORS GRUP de SENSORS i BIOSENSORS -- TECNOLOGIASTECNOLOGIAS
MEMBRANASPOTENCIOMETRICAS
TRANSDUCTORESOPTICOS ELECTRICOS
SPR SENSORES FIBRA OPTICA
SENSORES OPTO-
ELECTRONICOS
TRANSISTORES DE EFECTO DE CAMPO
(ISFET)
ELECTRODOS SELECTIVOSDE IONES
SENSORES AMPEROMETRICOS
MEMBRANASOPTICAS
TECNOLOGIA MICROELECTRONICA
POLIMEROS YCOMPOSITES
MATERIALESBIOCOMPOSITES
TECNOLOGIABIOQUIMICA
ENZIMAS
ANTICUERPOS
INMUNOSENSORESINMUNOSENSORES
TECNOLOGIACONVENCIONAL
ADN
GENOSENSORESGENOSENSORES
ADITIVOSIONOFOROSCROMOFOROS
QUÍMICA
SENSORES IMPEDIMETRICOS
DISPOSITIVOS 1 SOLO USO
(SERIGRAFIA)
DISPOSITIVOS 1 SOLO USO
(SERIGRAFIA)
NANOCOMPÓSITOSNANOCOMPÓSITOS
NANOPARTICULASNANOPARTICULAS
NANOTUBOS DE CNANOTUBOS DE C
SISTEMAS BIOMIMETICOS
LENGUAS ELECTRONICAS
POTENCIOMETRICAS
LENGUAS ELECTRONICAS
VOLTAMPEROMETRICAS
INFORMATICA
BIOSENSORESBIOSENSORESSENSORES QUIMICOSSENSORES QUIMICOS
(BIO)ANALIZADORES(BIO)ANALIZADORES
SISTEMAS ANALITICOSINTEGRADOS
(BIO) ANALIZADORES
AUTOMATIZADOS
FIA
SIA
WILSON & WILSON’SCOMPREHENSIVE
ANALYTICAL CHEMISTRYEDITED BY
D. BARCELÓ
VOLUME XXXIX
INTEGRATED ANALYTICAL SYSTEMS
BY
S. ALEGRET, Ed.
ELSEVIER
AMSTERDAM LONDON NEW YORK OXFORD PARIS SHANNON TOKYO
NANO BIO
INFO
COGNO
CONVERGING TECHNOLOGIES
NANO-BIO-INFO-COGNITIVE SYSTEMS
NANO-BIO-ANALYTICAL SYSTEMS
1. ION-SELECTIVE ELECTRODES (ISE)PVC membrane / graphite-polymer composite
Tubular electrodes Integrated flow-continuous systems
2. ION-SELECTIVE FIELD EFFECT TRANSISTORS (ISFET)
Chip (micro) Probe (meso) Gauge (macro)
3. ANALYSERS FOR ENVIRONMENTAL MONITORING BASED ON THE FIA TECHNIQUE (FLOW INJECTION ANALYSIS) AND ON POTENTIOMETRIC DETECTION
In collaboration with ADASA Sistemas, S.A
4. NETWORK OF AUTOMATIC STATIONS FOR WATER QUALITY CONTROL
Desarrollo y evaluación de analizadores automáticos para el control de parámetros de interés medioambiental en aguas
Estación Automática de Control de la Calidad
RÍO LLOBREGAT EN CASTELLBELL
RIERA DE RUBÍ EN RUBÍ
RÍO ANOIA EN MARTORELL
RED AUTOMÁTICA DE MONITORIZACIÓN DE LA CALIDAD DE LAS AGUAS (XACQA)
GÁLLEGO EN ZARAGOZA
EBRO EN MENDAVIA
ZADORRA EN ARCE
IREGUA EN ISLALLANA
EBRO EN MIRANDA
RED AUTOMÁTICA DE MONITORIZACIÓN DE LA CALIDAD DE LAS AGUAS (SAICA-EBRO)
VENTANAS PARA LA INTEGRACION DE DETECTORES OPTICOS Y TIPO ISFET
5. MICROSISTEMAS DE ANALISIS TOTALTecnología de silicio
ANALIZADOR DE ION PLOMO CON DETECCION POTENCIOMETRICA
MICROSISTEMAS DE ANALISIS TOTAL
Carrier/Sample
Conditioning solution
Cl- solution
Waste
Hydraulic circuit
log (ppmPb2+)
1,5 2,0 2,5 3,0 3,5 4,0 4,5
E (m
V)
10
20
30
40
50
60
70
80
90
E= -69 + 33.log(ppmPb2+ + 367)
Reference electrode
Connections
Ground
ISEGround
a
b
c
d
ISE connection to external set-up
Tecnología LTCC
MICROSISTEMAS DE ANALISIS TOTAL
ANALIZADOR DE ION NITRITO CON DETECCION COLORIMETRICA
2.2cm
2.6c
m
DISPOSITIVO LTCC
Ventana Óptica
Imagen RX
Time (s)
0 20 40 60 80 100 120 140 160 180
Abso
rban
ce
-0,1
0,0
0,1
0,2
0,3
0,4
0,5
0,6
0.1ppm0.5ppm1ppm2ppm5ppm7ppm10ppm
[NO2-] (ppm)
0 2 4 6 8 10 12
AB
SOR
BAN
CIA
0.0
0.5
1.0
1.5
2.0
2.5
Q=5.83µl/svolumen muestray reactivo=29µlRango 0.1-10ppmr2=0.9959
Abs=-3.31·10-3+0.048·[NITRITO]
6. INTEGRATED WAVEGUIDE ABSORBANCE OPTODES ACTIVATED WITH ION-SELECTIVE MEMBRANESIn collaboration with the National Microelectronics Centre of Barcelona (CNM)and the Optical Fibers Group from the University of Zaragoza
input Rib ARROW waveguide 10x4 micronsminimum losses 600-800 nmIsolating cladding
cut waveguide zonecore eliminationmembrane deposition 4 micronsoutput Rib ARROW waveguide50x4 micronsno cladding
7. ELECTROCHEMICAL IMMUNOSENSING BASED ON AFFINITY PLATFORMGraphite-polymer biocomposite
8. ELECTROCHEMICAL IMMUNOSENSING BASED ON mag-GEC
The competitive assay is performed in a solution containing:1.- Antibody modified magnetic particles
2.- Spiked sample
3.- Competitor Tracer (Tx-HRP)
Tx
TX-HRP
TxTxTx-HRPTx-HRP
Magnetic Particles
Size (μm)
Surface Active Group
Antibody Coupling Strategy
MP-COOH 0.196
Covalent Binding
MB-Tosyl
2.8
Covalent Binding
MB-ProtA
2.8
(Protein A)
Affinity Binding
For atrazine determination, specific antibodies were covalently coupled to MB-Tosyl, MP-COOH and MB-ProtA magnetic beads. For sulfonamides specific antibodies, Abs were covalently coupled to MB-Tosyl magnetic beads.
Antibiotics
Pesticides
Pollutants
Antibiotics
Pesticides
Pollutants
Magnetic beads / graphite-polymer composite
AA
CC
BB
M-GEC
streptavidin
biotinylatedprobe
HRP-antiDig
mismatch DNA
digoxigenin probe
magnet
magnetic bead
m-GEC electrode surface
A) Magnetic beads incubations occurs in solution (hybridization of DNA, enzyme or antibody labeling) B) The capture of the modified magnetic beads on the magneto sensor is produced by the magnetic affinity with the magnet inside the sensor body and C) Schematic representation of the electrochemical strategy based on m-GEC electrode.
ne-
HQ
HRP
H2O2H2O
DNA target
AA
CC
BB
M-GEC
streptavidin
biotinylatedprobe
HRP-antiDig
mismatch DNA
digoxigenin probe
magnet
magnetic bead
m-GEC electrode surface
A) Magnetic beads incubations occurs in solution (hybridization of DNA, enzyme or antibody labeling) B) The capture of the modified magnetic beads on the magneto sensor is produced by the magnetic affinity with the magnet inside the sensor body and C) Schematic representation of the electrochemical strategy based on m-GEC electrode.
ne-
HQ
HRP
H2O2H2O
DNA target
33
11 22
44
55
2 µm 10 µm
100 µm50 µm500 µm
33
11 22
44
55
2 µm 10 µm
100 µm50 µm500 µm
Scanning electron microphotographs showing the captured magnetic beads on the surface of m-GEC magneto sensor. Resolution: 2 µm (1), 10 µm (2), 50 µm (3), 100 µm (4) and 500 µm (5). Acceleration voltage: 15 kV.Number of magnetic beads: 6.2 x 106.
ELECTROCHEMICAL IMMUNOSENSING STRATEGY BASED ON mag-GECMagnetic beads / carbon-polymer composite
DIRECT VOLTAMMETRIC DETECTION
PhysicalAdsorption
open circuit
AuIII+ AuIII+AuIII+
Electrochemical oxidation0.1 M HCl
+1.25 V (vs. Ag/AgCl), 120 s
ElectrochemicalReduction
CV (+1.25 V to 0 V)
Cleaning+1.25 V
Au0 Au0
Au0Au0
Au0
M.Pumera, C.Mills, A.Merkoçi, S.Alegret; Electrochimica Acta, 2005, (published in Web )
DIRECT VOLTAMMETRIC DETECTION
PhysicalAdsorption
open circuit
AuIII+ AuIII+AuIII+
Electrochemical oxidation0.1 M HCl
+1.25 V (vs. Ag/AgCl), 120 s
ElectrochemicalReduction
CV (+1.25 V to 0 V)
Cleaning+1.25 V
Au0 Au0
Au0Au0
Au0
M.Pumera, C.Mills, A.Merkoçi, S.Alegret; Electrochimica Acta, 2005, (published in Web )
9. ELECTROCHEMICAL GENOSENSING BASED ON GOLD NANOPARTICLES
(A) introduction of streptavidin coated paramagnetic beads(B) immobilization of the biotinylated probe (DNA2) onto the paramagnetic beads(C) addition of the 1:1 Au67-DNA1 target(D) accumulation of Au67-DNA1/DNA2-paramagnetic bead conjugate on the surface of magnetic electrode(E) magnetically trigged direct DPV electrochemicaldetection of gold quantum dot tag in Au67-DNA1/DNA2-paramagnetic bead conjugate.
Langmuir, 21, 9625-9629 (2005)
Au
A B CAu
AuAu
Au
E
Au
AuAu
AuAu
AuAu
Au
D
DNA2 DNA1
Direct electrochemical detection of magnetically trigged DNA hybridization based Au67 – DNA – paramagnetic bead conjugate
GOLD NANOPARTICLES. Direct electrochemical detection
Au-NPs (Ø < 50 nm) / MBs (Ø < 10 µm)
10. ELECTROCHEMICAL SENSORS BASED ON CARBON NANOTUBESCNT-polymer composite
CNTs as surface modifiers
CNT composites
Carbon nanotubes integration in sensors
, ,
CNTs as surface modifiers
CNT composites
Carbon nanotubes integration in sensors
,
SistemasbiomimèticosSistemasbiomimèticos
11. Matrices de sensores11. Matrices de sensores
Matriz de sensorese-nosee-tongue
Colaboraciones requeridas
Validación interlaboratorios de los prototipos desarrolladosen muestras realesDesarrollo de instrumentación analítica
