Cleaning(oil(by(conver/ng(it(to(CO2(&(water(

One(step(Cleaning(Process

About(Oil(Spill(Eater(Interna/onal(

US(company.(Since(1989,(OSEI(is(responsible(for(the(clean(up(of(over(27,000(spills.((OSE(II(product(focuses(on(speeding(up(mother(nature(and(is(environmentally(friendly.(

Cleanup(of(spills(including(brackish(water,(ocean(water,(fresh(water(and(even(interJ/dal(areas(just(to(name(a(few.((

• US(Department(Of(Defense(• US(Navy(• US(Coast(Guard(• OSE(II(is(used(in(more(than(195(US(bases

(both(US(based(&(globally)(

Oil(Spill(Eater(IIJMechanism(of(Ac/on

These(illustra/ons(represent(a(simplified(overview(of(how(OSE(II(breaks(down(oil(

and(converts(it(to(CO2(and(water

When(OSE( II( is( applied( to( an( oil( spill( on( open(water,( the(molecular( structure(begins(to(breakdown(immediately(and(in(a(short(/me,(visually(disappear.(This(is(caused( through( the( ac/on( of( bio( surfactants( that( connect( with( the( oil(molecules(and((breakdown(the(covalent(and(ionic(bonds(crea/ng(small(micelles((oil(droplets).((This(process(rapidly(reduces(the(toxicity(of(the(oil(making(it(more(bioavailable(to(indigenous(bacteria(that(u/lise(the(oil(as(a(food(source.(

OSE(II(Stage(1

Enzyme

Oil(Droplet(or(micelle

Oil(Before(applica/on(of(OSE(II

Oil(star/ng(to(break(up(immediately(when(OSE(II(is(applied

Oil(breaking(down(to(very(small(droplets(or(micelles(aZer(a(short(/me(once(OSE(II(is(applied

Oil(droplets(are(virtually(invisible(in(a(short(/me(aZer(applying(OSE(II(

enzyme

Once(OSE( II( has( come( into( contact(with( oil(molecules,( the( enzymes( form(protein( binding(sites(enabling(the(already(present(bacteria(to(u/lise(the(oil(as(a(food(source.(OSE(II(enzymes(and(biosurfactants(create(a(detoxified(medium(of(smaller(separated(droplets(called(micelles,(where(indigenous(bacteria(can(safely(a[ach(themselves(and(digest(to(CO2(and(water(.((OSE(II(also(changes( the(density(of( the(oil(making( it(float(and(reduces( the(adhesion(proper/es(so(the(oil(will(no(longer(adhere(to(shorelines,(vegeta/on(or(man(made(structures.

OSE(II(Stage(2

OSE( II( contains( nutrients( that( a[ract( indigenous(bacteria( that( rapidly(colonize/grow( in(numbers(greatly(speeding(up(spill( remedia/on.(OSE( II(does(not(contain(any(foreign(bacteria(or(non( indigenous(organisms.(The(bacteria,( natural( to( ( the( spill( environment,( rapidly(consume(detoxified(oil(with(the(help(of(OSE(II(nutrients(and( enzymes( un/l( all( is( converted( to( CO2( and(water,(permanently(removing(the(oil(from(the(environment.

NATURAL(BACTERIA(LIVING(IN(THE(SPILL(ENVIRONMENT(DIGEST(THE(OIL(TO(100%(NON(DETECT(

OIL SPILL EATER INTERNATIONAL, CORP.

P.O. Box 515429Dallas, Texas 75075Ph: (972) 669-3390Fax: (469) 241-0896Email: oseicorp@msn.comWeb: www.osei.us

TPH

2,000

1,800

1,600

1,400

1,200

1,000

800

600

400

200

020 30 40 50 60 70 80 DAYS

SOUND ANALYTICAL SERVICESTACOMA, WASHINGTON

60 YEAR OILED SOIL Tacoma, Washington Sept-Nov 1990

Start 1,800 TPHEnd 10 TPH

TPH

100,000

9,000

8,000

7,000

6,000

5,000

4,000

3,000

2,000

1,000

010 20 30 40 50 60 DAYS

SAVANNAH LABORATORIESATLANTA, GEORGIA

WEATHERED VENEZUELAN

Start 105,000 TPHEnd 41 TPH

Atlanta, GeorgiaJune 1990

TPH

1,000

900

800

700

600

500

400

300

200

100

01 2 3 4 5 6 7 8 DAYS

SOUTHERN TESTING LABS.ANCHORAGE, ALASKA

OILED SOIL

Start 767 TPHEnd 391 TPH

Anchorage, AlaskaMarch 1990

TPH

100,000

9,000

8,000

7,000

6,000

5,000

4,000

3,000

2,000

1,000

020 30 40 50 60 70 80 90 DAYS

90 YEAR OILED SOIL

Start 9,300 TPHEnd 2,600 TPH

Tacoma, WashingtonBulk Fuel TerminalSept-Nov 1990

SOUND ANALYTICAL SERVICESTACOMA, WASHINGTON

TPH

100,000

9,000

8,000

7,000

6,000

5,000

4,000

3,000

2,000

1,000

050 100 150 200 250 HOURS

SOUTHWESTERN LABORATORIESSAN ANTONIO, TEXAS

LIGHT TO MEDIUM CRUDE

Start 106,00 TPHEnd 529 TPH

MEGA BORG.Gulf of MexicoJune 1990

TPH

1,000

900

800

700

600

500

400

300

200

100

05 10 15 20 25 30 DAYS

MEDIUM TO HEAVY CRUDE

Start 1,000 TPHEnd 89 TPH

Prudoe Bay, AlaskaJune 1990

CHEMICAL ANALYSIS, INC.DALLAS, TEXAS

TPH BIODEGRADATION TESTS OF OSE IIPERFORMED BY INDEPENDENT LABORATORIES

This page represents a sampling of laboratory test reports available upon request.

24

https://www.youtube.com/watch?v=o8XbOKicZ_M

https://youtu.be/dJcq3ONnESM

An/JPollu/on( Tank(Cleaning

Deck(Cleaning(No(problem(if(it(goes(

overboard,(EPA(approvedEngine(room(cleaning

Engine(room(cleaning

An/Jpollu/on(preven/on(during(bunkering

Hard(to(reach(areas(cleaning

Terrain(Cleaning

Industrial(Applica/on(Cleaning

Underground(Spill(Treatment

Tank(water(oil(contamina/on(treatment

Fire(Retardancy(Proper/es

Thank(You(