monografia pululanasas y pectinasas

7/25/2019 monografia pululanasas y pectinasas

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UNIVERSIDAD AUTNOMA DE COAHUILA

FACULTAD DE CIENCIAS BIOLGICAS

Maestra en Ing. Bioqui!a

Monografa de pectinasas y pululanasas

IN"ENIERIA EN#IMATICA

CATEDR$TICO% DR. MIRIAM &AULINA LUEVANOS ESCARE'O

TORREN( COAHUILA)* DE NOVIEMBRE DEL )+,-


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ndice

n/i!e /e 0iguras 1

n/i!e /e ta23as 1

Resuen 4A2stra!t 4

Intro/u!!i5n -

O26eti7os 8

, &e!tinasas *

,., Ante!e/entes *,.,., Historia *

,.,.) C3asi0i!a!i5n 9,.,.1 Re3e7ante ,+,.) :uentes /e o2ten!i5n ; ti


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Re0eren!ias 2i23iogr>0i!as 1+

ndice figuras

:ig. ,. A!!i5n en?i>ti!a /e 3as


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Resumen

Las


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Introduccin

Las en?ias 0ueron /es!u2iertas en 3a segun/a ita/ /e3 sig3o I ; /es/e enton!es @an

si/o /e gran uti3i/a/


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Objetivos

Co


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1. Pectinasas1.1. Antecedentes

1.1.1. Historia

Las


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ent este

Las


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1ndo+olimetilgala$t#ronato liasa Pe$tin liasa 4.2.2.: Pe$tina /andom

1o+olimetilgala$t#ronato liasa Pe$tin liasa Pe$tina )erminal

Las


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Las /e


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3as sustan!ias s a!!esi23es. Otras !ar2o@i/ro3asas a


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Las !e


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Una eo


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in7estiga!iones @an ino7i3i?a/o /e en/o


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Las


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1o!+e$tinasa 3.5!5.:Penicilliumfrequentans 1ndo!P" 4.5!4. 5:

Sclerotium rolfsii 1ndo!P" 3.5 55

Rhizoctonia solani 1ndo!P" 4. 5:

Mucor pusilus P" 5.: 4:Clostridiumthermosaccharolyticum P"8 5.5!.: 3:!4:

Pe$tinas al$alinas

Bacillus sp. /(9 P"8 :.: !

Bacillus sp.=)!33 P" :.5 5

Bacillus polymyxa P" .4!9.4 45

Bacillus pumilis PA)1 .:!.5 6:

Amucola sp. PA8 :.25 :Xanthomonascampestris PA)1 9.5 25!3:

Bacillus =o. P!4!= P" :!:.5 65

Bacillusstrarothermophilus PA)1 9.: :

Penicillium italicumC1C) 2294 Pe$tin liasa .: 5:

Bacillus sp.') Pe$tin liasa .: 6:

Bacillus sutilis Pe$tato liasa .5 6:!65

Pseudomonassyringae p!."lycinea Pe$tato liasa .: 3:!4:

&ara 3a


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2. Pululanasas2.1. Antecedentes2.1.1. Historia

La en?ia


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2.1.2. Clasificacin

Las 3isis ; 3a es


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2.1.. Relevante

La @a2i3i/a/ /e 3as


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2.2. !uentes de obtencin " ti#os

La


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:ig. 4. Degra/a!i5n /e3


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"3u!onato( 3os !ua3es se en!uentran en e3 sitio !ata3ti!o /e 3a en?ia. Este nue7o ti


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"l#$oamilasa (la!oacterioum sp 5.5!6.5 !!!!!!!!Aso$iada a$l#la

"l#$oamilasa )aloacterium sodomense .5 !!!!!!!!

"l#$oamilasa 'hermomyces lanuginosus 4.9 !!!!!!!! 1tra$el#l

&or 3tio 3as g3u!oai3asas son eog3u!anasas( 3as !ua3es /e anera !onse!uti7a ro


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La uti3i?a!i5n /e 3a


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Los estu/ios o3e!u3ares en 3os genes que !o/i0i!an


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es /e 0>!i3 0erenta!i5n(


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&iscusiones

Las s se uti3i?an en 3a in/ustria a3ientos( to/os

3os autores sePa3an que 3as


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Conclusin

A2as en?ias


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Referencias bibliogr'ficas

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ma$erans. (-MS Microiology etters* $5, 269!23.Al&orta, I., "aris#, C., 8lama, >. *., Serra, *. 8. 99. Ind#strial a++li$ations

oD +e$ti$ enzymesE a re%ie-. Process Biochemistry* ++, 2!2.Arellano!Cara., Hoondal, ". 2:::. Prod#$tion and

$hara$terization oD thermostale ylanase and +e$tinase DromStre+tomy$es s+. "!!3./ournal of industrial microiology 1iotechnology* 206, 396!4:2.

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on +rod#$tion oD ylanase and +e$tinase Drom Stre+tomy$es s+. "!!3.3orld /ournal of Microiology and Biotechnology* $42, 2!23.

Brinton, C., 0i$hmann, H., 0illaman, *., 0ilson, C., 'ore, 0. 92.'enitions -ritten y the Committee on =omen$lat#re oD Pe$tin oD theAgri$#lt#re!ood 'i%ision./. Am. Chem. Soc* 05, 3!4:.

Carrera, *. 1. 2::3. Prod#$$iJn y a+li$a$iJn de enzimas ind#striales (acultadde Ciendias Agropecuarias* $.

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+e$tinase in Dr#it ., 8a#s, >., 8anzarini, ". 996. /e%ersile adsor+tion oDendo+e$tin!lyase to tailor!made $ore!shell mi$ros+heres +re+ared ydis+ersion +olymerization. Biotechnology and applied iochemistry*2+2, 33!4:.

'oman!Pyt&a, >., Bardo-s&i, *. 2::4. P#ll#lan degrading enzymes oDa$terial origin. Critical re!ie7s in microiology* +82, :!2.

'#ner, ., Bertoldo, C., Andersen, *. )., 0agner, (., Antrani&ian, ". 2:::. Ane- thermoa$ti%e +#ll#lanase Drom 'es#lD#ro$o$$#s m#$os#sE $loning,seO#en$ing, +#ri$ation, and $hara$terization oD the re$ominantenzyme aDter e+ression in Ba$ill#s s#tilis./ournal of acteriology*

$9222, 633!633."erhartz, 0. 99:. -nzymes in industry: production and applicationsE $h

erlagsgesells$haDt mH."#mmadi, S. =., Panda, ). 2::3. P#ri$ation and io$hemi$al +ro+erties oD

mi$roial +e$tinasesQa re%ie-. Process Biochemistry* +9, 9!996.Hayashi, (., Ino#e, R., Shiga, >., Sato, S.!I., )a&ano, /., Hirayae, (., . . . Hara, S.

99. Pe$tinolyti$ enzymes Drom Pse#domonas marginalis >A :3!:3. Phytochemistry* 0;, 359!363.

3:


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Heinri$ho%L, (., lie$ho%$o%L, '. 96. Poly ethylene tere+hthalateimmoilized eo!'!gala$t#ronanase. Collection of Czechoslo!aanenti, I., 8F P/1S)I, A., S+agna, ". 99. Immoilization oD+e$tinesterase on y!al#mina Dor the treatment oD Dr#it ode oDa$tion oD endo+olygala$t#ronase immoilized y adsor+tion and y$o%alent inding %ia amino gro#+s. Biotechnology and ioengineering*+0, 9!5.

Saha, B. C., eis, *. ". 99. =o%el highly thermostale +#ll#lanase Dromthermo+hiles. 'rends in Biotechnology* ,9, 234!239.

Sa&ai, )., Sa&amoto, )., Hallaert, *., andamme, 1. *. 993. Pe$tin, +e$tinaseand +roto+e$tinaseE +rod#$tion, +ro+erties, and a++li$ations.Ad!ancesin applied microiology* +5, 23.

Sier&s, >. /., ord, C., /eilly, P. *., S%ensson, B. 99. Site!dire$tedm#tagenesis at the a$ti%e site )r+l2: oD As+ergill#s a-amorigl#$oamylase. Protein engineering* 2, 62!625.

Sola&, A. I., /i$hardson, ). H., >$Cann, /. )., (line, (. A., Bartne&, .,)omlinson, "., . . . Podar, >. 2::5. 'is$o%ery oD +e$tin!degradingenzymes and dire$ted e%ol#tion oD a no%el +e$tate lyase Dor +ro$essing$otton Dari$./ournal of Biological Chemistry* 298:, 943!943.

)a&asa&i, R. 96. P#riDi$ations and 1nzymati$ Pro+erties oD Y!Amylase andP#ll#lanase Drom Ba$ill#s $ere#s %ar. my$oides.Agricultural and

Biological Chemistry* 08, 523!53:.)a&iza-a, =., >#roo&a, R. 95. Cloning oD the +#ll#lanase gene and

o%er+rod#$tion oD +#ll#lanase in 1s$heri$hia $oli and (lesiellaaerogenes.Applied and en!ironmental microiology* 052, 294!29.

)onoz#&a, )., >ogi, S.!i., Shim#ra, R., I#&a, A., Sa&ai, H., >ats#za-a, H., . . .Fhta, ). 995. Com+arison oD +rimary str#$t#res and s#strates+e$iDi$ities oD t-o +#ll#lan!hydrolyzing ?!amylases, )A I and )A II,Drom )hermoa$tinomy$es %#lgaris /!4. Biochimica et Biophysica Acta@BBA#Protein Structure and Molecular -nzymology* $2;2, 35!42.

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hang, 8., h#, @., heng, S., S#n, H. 2::9. Photo$hemi$al +re+aration oDmagneti$ $hitosan eads Dor immoilization oD +#ll#lanase. Biochemical-ngineering /ournal* 04, 3!.

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