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\fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li6800\ri0\sl-506\slmult0 \fs42\cf0\f0\charscalex100\b \i {Diciembre de 2007 }\b0 \i0 \par\pard\sect\sectd\sbkpage{\shp{\*\shpinst\shpleft1386\shptop1040\shpright10853\shpbottom1093\shpfhdr0\shpwr3\shpwrk0\shpfblwtxt1\shplid2031\shpz0\shpbxpage\shpbypage{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{\sn rotation}{\sv 0}}{\sp{\sn geoRight}{\sv 9467}}{\sp{\sn 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\fs18\cf0\par\pard\li746\ri0\sl-520\slmult0 \fs36\cf0\f0\charscalex100\b \i {Captulo 1. }\b0 \i0 \par\column\pard\li0\ri0\sl-320\slmult0 \fs24\cf0\f0\charscalex100\b \i {Introduccin a los mtodos de separacin }\b0 \i0 \par\pard\sect\sectd\sbknone\pgwsxn12240\pghsxn15840\marglsxn666\margrsxn133\pard\li746\ri0\sl-600\slmult0 \fs36\cf0\f0\charscalex100\b \i {Introduccin a los mtodos de separacin }\b0 \i0 \par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-346\slmult0 \fs30\cf0\f0\charscalex100\b \i {1.1 Introduccin a la cromatografa }\b0 \i0 \par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-293\slmult0 \fs22\cf0\f1\charscalex100 {En 1906, el botnico Ruso M. Tswett realiz un experimento que condujo al descubrimiento de lo }\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {que hoy conocemos como }{ }\fs24\cf0\f0\charscalex100\b \i {cromatografa}\b0 \i0 \fs24\cf0\f1\charscalex100 {. Coloc un extracto de pigmentos vegetales en la parte }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {superior de una columna de vidrio rellena de carbonato de calcio (CaCO}\fs14\cf0\f1\charscalex100 {3}\fs22\cf0\f1\charscalex100 {). Al agregar ter, observ que la }\par\pard\li746\ri0\sl-413\slmult0 \fs24\cf0\f1\charscalex100 {mezcla original se separaba en diversas bandas coloridas que descendan a travs de la columna a }\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {diferentes velocidades. }\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-306\slmult0 \fs20\cf0\f1\charscalex100 {Un rasgo caracterstico de la cromatografa es la presencia de dos fases; dispuestas de tal manera }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {que mientras una permanece estacionaria dentro del sistema (fase estacionaria), la otra se desplaza a lo }\par\pard\li746\ri0\sl-413\slmult0 \fs22\cf0\f1\charscalex100 {largo de l (fase mvil). La clave de la separacin en cromatografa es que la velocidad con la que se }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {mueve cada sustancia depende de su afinidad relativa por ambas fases (equilibrio de distribucin). En el }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {experimento de Tswett, la separacin de los pigmentos vegetales se logr gracias a que cada uno de }\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {ellos tena una afinidad diferente por las fases. En general, los componentes ms afines a la fase }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {estacionaria avanzan lentamente (ms retenidos) mientras que los ms afines a la fase mvil (menos }\par\pard\li746\ri0\sl-413\slmult0 \fs22\cf0\f1\charscalex100 {retenidos) se mueven con mayor rapidez. Por consecuencia, el medio cromatogrfico (columna, placa o }\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {papel) funciona como un controlador de la velocidad de cada sustancia que constituye la mezcla}\fs24\cf0\f1\charscalex100 {,}\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {logrando as su separacin }\fs22\cf0\f1\charscalex100 {y }\fs22\cf0\f1\charscalex100 {mediante el uso de un detector, su caracterizacin qumica. }\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-306\slmult0 \fs24\cf0\f1\charscalex100 {Aunque los principios fundamentales son los mismos, se acostumbra clasificar los mtodos }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {cromatogrficos segn el estado fsico de la fase mvil: }\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-320\slmult0 \fs22\cf0\f0\charscalex100\b \i {Cromatografa lquida}\b0 \i0 \fs24\cf0\f1\charscalex100 {. La fase mvil es un disolvente o mezcla de disolventes y la fase estacionaria un }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {slido que interacta con las sustancias que se desea separar (}\fs20\cf0\f0\charscalex100\b \i {cromatografa lquido-slido}\b0 \i0 \fs22\cf0\f1\charscalex100 {), o bien un }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {lquido inmiscible con la fase mvil, depositado en la superficie de un slido (}\fs22\cf0\f0\charscalex100\b \i {cromatografa lquido-}\b0 \i0 \par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f0\charscalex100\b \i {lquido}\b0 \i0 \fs24\cf0\f1\charscalex100 {). Esta forma de cromatografa puede realizarse con diferentes arreglos experimentales: en }\par\pard\li746\ri0\sl-413\slmult0 \fs22\cf0\f1\charscalex100 {columna, en capa delgada o en papel. En el primer caso, la fase estacionaria se encuentra rellenando un }\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {tubo; en el segundo, se dispersa sobre una lmina de vidrio o aluminio formando un lecho de espesor }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {uniforme; en la cromatografa en papel, la fase estacionaria es la solucin acuosa contenida en }\fs22\cf0\f1\charscalex100 {el}\fs22\cf0\f1\charscalex100 { interior }\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {de las celdas formadas por las fibras de la celulosa, y es por tanto una forma de cromatografa lquido-}\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {lquido. }\par\pard\sect\sectd\sbknone\pgwsxn12240\pghsxn15840\marglsxn666\margrsxn133\cols2\colno1\colw10040\colsr-0\colno2\colw1400\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-293\slmult0 \fs24\cf0\f2\charscalex100 {Captulo 1 }\par\column\pard\li0\ri0\sl-213\slmult0 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slido inerte}\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {(}\fs20\cf0\f0\charscalex100\b \i {cromatografa g}\b0 \i0 \fs20\cf0\f0\charscalex100\b \i {as}\b0 \i0 \fs20\cf0\f0\charscalex100\b \i {-lquido}\b0 \i0 \fs22\cf0\f1\charscalex100 {). Este tipo de cromatografa siempre es en columna, ya que es la nica }\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {manera de que la fase mvil gaseosa se mantenga fluyendo, confinada dentro del sistema. La columna}\par\pard\li746\ri0\sl-400\slmult0 \fs20\cf0\f1\charscalex100 {puede estar rellena con la fase estacionaria, en forma semejante a la cromatografa lquida, o bien la fase }\par\pard\li746\ri0\sl-413\slmult0 \fs20\cf0\f1\charscalex100 {estacionaria puede depositarse sobre las paredes de un tubo muy delgado (0.25mm de dimetro) y largo }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {(hasta 100m). Este tipo de columnas se conocen como columnas capilares y proporcionan la mayor }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {capacidad de separacin. }\fs22\cf0\f1\charscalex100 {De}\fs22\cf0\f1\charscalex100 { acuerdo con el mecanismo de retencin, la cromatografa se puede }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {clasificar en los siguientes tipos: }\par\pard\sect\sectd\sbknone\pgwsxn12240\pghsxn15840\marglsxn666\margrsxn133\cols2\colno1\colw6173\colsr-0\colno2\colw5266\pard\li1106\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1106\ri0\sl-320\slmult0 \*\tx1466\fs24\cf0\f3\charscalex100 {\uc1\u61623X}\tab \fs22\cf0\f1\charscalex100 {adsorcin (normal, de fase reversa) }\par\pard\li1106\ri0\sl-426\slmult0 \*\tx1466\fs24\cf0\f3\charscalex100 {\uc1\u61623X}\tab \fs22\cf0\f1\charscalex100 {particin lquido-lquido }\par\pard\li1106\ri0\sl-413\slmult0 \*\tx1466\fs24\cf0\f3\charscalex100 {\uc1\u61623X}\tab \fs22\cf0\f1\charscalex100 {intercambio inico }\par\column\pard\li0\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li0\ri0\sl-320\slmult0 \*\tx360\fs24\cf0\f3\charscalex100 {\uc1\u61623X}\tab \fs24\cf0\f1\charscalex100 {permeacin sobre gel }\par\pard\li0\ri0\sl-426\slmult0 \*\tx360\fs24\cf0\f3\charscalex100 {\uc1\u61623X}\tab \fs24\cf0\f1\charscalex100 {de afinidad }\par\pard\sect\sectd\sbknone\pgwsxn12240\pghsxn15840\marglsxn666\margrsxn133\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {Las reas de aplicacin son muy diversas y abarcan prcticamente todas las actividades en las que }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {interviene la qumica, por ejemplo: se emplea en: }\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-306\slmult0 \fs22\cf0\f1\charscalex100 {El anlisis de drogas y frmacos en fluidos biolgicos como la saliva, la sangre, la orina; }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {Seguir la transformacin de las sustancias responsables de la transmisin neurolgica; }\par\pard\li746\ri0\sl-413\slmult0 \fs22\cf0\f1\charscalex100 {Determinar la presencia de contaminantes en el medio ambiente; }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {Descifrar la composicin de los combustibles fsiles; }\par\pard\li746\ri0\sl-400\slmult0 \fs20\cf0\f1\charscalex100 {Realizar el control de calidad de los productos qumicos y farmacuticos manufacturados; en fin, la lista }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {de ejemplos es interminable. }\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-293\slmult0 \fs30\cf0\f0\charscalex100\b \i {1.2}\b0 \i0 \fs30\cf0\f0\charscalex100\b \i {Cromatografa en papel }\b0 \i0 \par\pard\li1466\ri0\sl-440\slmult0 \fs22\cf0\f1\charscalex100 {La cromatografa en papel es un proceso muy utilizado en los laboratorios para realizar {\field{\*\fldinst{HYPERLINK "http://es.wikipedia.org/w/index.php?title=An%C3%A1lisis_cualitativo&action=edit"}}{\fldrslt {anlisis }}}}\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {{\field{\*\fldinst{HYPERLINK "http://es.wikipedia.org/w/index.php?title=An%C3%A1lisis_cualitativo&action=edit"}}{\fldrslt {cualitativos }}}ya que pese a no ser una tcnica potente no requiere de ningn tipo de equipamiento. La }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {fase estacionaria est constituida simplemente por una tira de papel de filtro. La muestra se deposita en }\par\pard\li746\ri0\sl-413\slmult0 \fs22\cf0\f1\charscalex100 {un extremo colocando pequeas gotas de la }\fs22\cf0\f1\charscalex100 {di}\fs22\cf0\f1\charscalex100 {solucin y evaporando el }\fs22\cf0\f1\charscalex100 {di}\fs22\cf0\f1\charscalex100 {solvente. Luego el {\field{\*\fldinst{HYPERLINK "http://es.wikipedia.org/wiki/Disolvente"}}{\fldrslt {disolvente }}}}\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {empleado como fase mvil se hace ascender por{\field{\*\fldinst{HYPERLINK "http://es.wikipedia.org/wiki/Capilaridad"}}{\fldrslt { capilaridad.}}} La separacin se realiza en funcin de la }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {afinidad de los solutos con las dos fases, las ms solubles en agua se quedarn cerca del punto donde se }\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {aplic la muestra, y las menos solubles en agua y ms solubles en el disolvente llegarn ms lejos. Las }\par\pard\li746\ri0\sl-413\slmult0 \fs22\cf0\f1\charscalex100 {sustancias separadas se identifican mediante diversos procedimientos fsicos o qumicos }\par\pard\sect\sectd\sbknone\pgwsxn12240\pghsxn15840\marglsxn666\margrsxn133\cols2\colno1\colw10040\colsr-0\colno2\colw1400\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-426\slmult0 \fs24\cf0\f2\charscalex100 {Captulo 1 }\par\column\pard\li0\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li0\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li0\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li0\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li0\ri0\sl-426\slmult0 \fs24\cf0\f2\charscalex100 {2 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\fs24\cf0\f0\charscalex100\b \i {Introduccin a los mtodos de separacin }\b0 \i0 \par\pard\li1120\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1120\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1120\ri0\sl-253\slmult0 \fs22\cf0\f1\charscalex100 {La cromatografa en papel es una tcnica utilizada para anlisis inorgnico cualitativo, permite llevar }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {a cabo la separacin e identificacin de iones, trabajando con cantidades mnimas de sustancia. }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {Pertenece al tipo de \uc1\u8220XCromatografa de particin\uc1\u8221X se fundamenta en que las sustancias problema, }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {pueden tener diferentes coeficientes de reparto en dos disolventes de inmiscibilidad limitada, uno }\par\pard\li746\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {permanece fijo en la superficie del papel \uc1\u8220Xfase estacionaria\uc1\u8221X generalmente en agua, la fase mvil}\par\pard\li746\ri0\sl-413\slmult0 \fs20\cf0\f1\charscalex100 {constituida generalmente por una mezcla de disolventes parcialmente miscibles en ella. Hay varios tipos }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {de cromatografa, la ascendente (papel hacia arriba), descendente (papel invertido), radial y de }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {separacin de zonas y sectores. }\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-413\slmult0 \fs20\cf0\f1\charscalex100 {Al entrar en contacto con los disolventes empieza su fase de movilidad lo que produce unas }\par\pard\li746\ri0\sl-400\slmult0 \fs20\cf0\f1\charscalex100 {manchas caractersticas sobre el papel, generalmente, estas no son coloreadas y se revelan con una }\par\pard\li746\ri0\sl-400\slmult0 \fs20\cf0\f1\charscalex100 {lmpara fluorescen}\fs20\cf0\f1\charscalex100 {te}\fs20\cf0\f1\charscalex100 {, los contornos se marcan con lpiz. Como medida en cromatografa sobre papel se }\par\pard\li746\ri0\sl-413\slmult0 \fs22\cf0\f1\charscalex100 {emplea el}{ }\fs22\cf0\f0\charscalex100\b \i {Rf}\b0 \i0 \fs22\cf0\f1\charscalex100 { (}\fs22\cf0\f0\charscalex100\i {Retention factor}\i0 \fs22\cf0\f1\charscalex100 {), el cual se define como el cociente de dividir el recorrido de la sustancia }\par\pard\li746\ri0\sl-400\slmult0 \fs20\cf0\f1\charscalex100 {por el disolvente, esto es, la distancia media desde el origen hasta el centro de la mancha (X) dividida por }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {la distancia que media desde el origen hasta el frente del disolvente (}\fs24\cf0\f0\charscalex100\b \i {S}\b0 \i0 \fs24\cf0\f1\charscalex100 {).}\fs24\cf0\f1\charscalex100 { }\par\pard\sect\sectd\sbknone\pgwsxn12240\pghsxn15840\marglsxn666\margrsxn133\cols2\colno1\colw6040\colsr-0\colno2\colw5400\pard\li4626\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li4626\ri0\sl-346\slmult0 \fs24\cf0\f2\charscalex100 {\uc1\u55349X\uc1\u56389X\uc1\u55349X\uc1\u56403X =}{ }\fs18\cf0\f2\charscalex100 {\uc1\u55349X\uc1\u56395X}\par\column\pard\li0\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li0\ri0\sl-360\slmult0 \fs24\cf0\f1\charscalex100 {[1] }\par\pard\sect\sectd\sbknone\pgwsxn12240\pghsxn15840\marglsxn666\margrsxn133\pard\li5186\ri0\sl-186\slmult0 \fs18\cf0\f2\charscalex100 {\uc1\u55349X\uc1\u56390X}\par\pard\li1466\ri0\sl-400\slmult0 \fs24\cf0\f1\charscalex100 {En la cromatografa en papel se utiliza como fase estacionaria una hoja de papel de celulosa de }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {elevada pureza recubierta de una capa de agua asociada a las fibras de celulosa. La fase mvil, en la que}\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {ir disuelta la muestra, }\fs22\cf0\f1\charscalex100 {se}\fs22\cf0\f1\charscalex100 { forma por disolventes cuya naturaleza se elige en funcin de los componentes }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {que se pretenden separar. }\par\pard\li746\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li746\ri0\sl-453\slmult0 \fs30\cf0\f0\charscalex100\b \i {1.3 Cromatografa en capa fina }\b0 \i0 \par\pard\li1466\ri0\sl-213\slmult0 \fs18\cf0\par\pard\li1466\ri0\sl-240\slmult0 \fs22\cf0\f1\charscalex100 {La cromatografa en capa fina se basa en la preparacin de una capa, uniforme, de un }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {adsorbente mantenido sobre una placa de vidrio u otro soporte. Los requisitos esenciales son, pues, un }\par\pard\li746\ri0\sl-400\slmult0 \fs22\cf0\f1\charscalex100 {adsorbente, placas de vidrio, un dispositivo que mantenga las placas durante la extensin, otro para }\par\pard\sect\sectd\sbknone\pgwsxn12240\pghsxn15840\marglsxn666\margrsxn133\cols2\colno1\colw10040\colsr-0\colno2\colw1400\pard\li746\ri0\sl-466\slmult0 \fs24\cf0\f2\charscalex100 {Captulo 1 }\par\column\pard\li0\ri0\sl-466\slmult0 \fs24\cf0\f2\charscalex100 {3 }\par\pard\sect\sectd\sbknone\pgwsxn12240\pghsxn15840\marglsxn666\margrsxn133\pard\sect\sectd\sbkpage{\shp{\*\shpinst\shpleft1386\shptop1040\shpright10853\shpbottom1093\shpfhdr0\shpwr3\shpwrk0\shpfblwtxt1\shplid2045\shpz0\shpbxpage\shpbypage{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{\sn rotation}{\sv 0}}{\sp{\sn geoRight}{\sv 9467}}{\sp{\sn geoBottom}{\sv 53}}{\sp{\sn shapePath}{\sv 4}}{\sp{\sn pVerticies}{\sv 8;5;(0,0);(0,66);(9466,66);(9466,0);(0,0);}}{\sp{\sn pSegmentInfo}{\sv 2;12;16384;45824;1;45824;1;45824;1;45824;1;45824;24577;32768}}{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 2303074}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineColor}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 0}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn fLayoutInCell}{\sv 1}}}}{\shp{\*\shpinst\shpleft1386\shptop1013\shpright10853\shpbottom1026\shpfhdr0\shpwr3\shpwrk0\shpfblwtxt1\shplid2046\shpz1\shpbxpage\shpbypage{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{\sn rotation}{\sv 0}}{\sp{\sn geoRight}{\sv 9467}}{\sp{\sn geoBottom}{\sv 13}}{\sp{\sn shapePath}{\sv 4}}{\sp{\sn pVerticies}{\sv 8;5;(0,0);(0,13);(9466,13);(9466,0);(0,0);}}{\sp{\sn pSegmentInfo}{\sv 2;12;16384;45824;1;45824;1;45824;1;45824;1;45824;24577;32768}}{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 2303074}}{\sp{\sn fLine}{\sv 1}}{\sp{\sn lineColor}{\sv 2303074}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 0}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn fLayoutInCell}{\sv 1}}}}{\shp{\*\shpinst\shpleft1386\shptop14493\shpright10853\shpbottom14560\shpfhdr0\shpwr3\shpwrk0\shpfblwtxt1\shplid2047\shpz2\shpbxpage\shpbypage{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{\sn rotation}{\sv 0}}{\sp{\sn geoRight}{\sv 9467}}{\sp{\sn geoBottom}{\sv 67}}{\sp{\sn shapePath}{\sv 4}}{\sp{\sn pVerticies}{\sv 8;5;(0,0);(0,66);(9466,66);(9466,0);(0,0);}}{\sp{\sn pSegmentInfo}{\sv 2;12;16384;45824;1;45824;1;45824;1;45824;1;45824;24577;32768}}{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 2303074}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineColor}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 0}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn fLayoutInCell}{\sv 1}}}}{\shp{\*\shpinst\shpleft1386\shptop14573\shpright10853\shpbottom14586\shpfhdr0\shpwr3\shpwrk0\shpfblwtxt1\shplid2048\shpz3\shpbxpage\shpbypage{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{\sn rotation}{\sv 0}}{\sp{\sn geoRight}{\sv 9467}}{\sp{\sn geoBottom}{\sv 13}}{\sp{\sn shapePath}{\sv 4}}{\sp{\sn pVerticies}{\sv 8;5;(0,0);(0,13);(9466,13);(9466,0);(0,0);}}{\sp{\sn pSegmentInfo}{\sv 2;12;16384;45824;1;45824;1;45824;1;45824;1;45824;24577;32768}}{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 2303074}}{\sp{\sn fLine}{\sv 1}}{\sp{\sn lineColor}{\sv 2303074}}{\sp{\sn lineType}{\sv 0}}{\