Seminario de autoformaciónCaso N° 04

“Uniones Intercelulares en cultivo endotelial”

MICROSCOPÍA ELECTRÓNICA 2015

Universidad de ChileFacultad de MedicinaDepto. de Tecnología médicaEscuela de Tecnología Médica

Resultados Esperados• Basal

Thin section of aortic endothelial cells. (a) Cultured cells. Note that the cells are thin, especially away from the nucleus. Nucleus has uniform chromatin. Interfaces with occasional junctions often involve overlapping of thin processes (arrow). Bar, 1 µm. (b) Effluent cells showing peripheral clumping of nuclear chromatin, swollen cristae, and dense matrix in mitochondria,numerous micropinocytotic vesicles, and groups of intermediate filaments. Each interface (arrow) typically has a junctional complex. Bar 1 µm.

Resultados Esperados• Basal

(c) Intact aortic segments. The upper endothelial layer is separated from underlying smooth muscle cells by a thin basal lamina and diffuse extracellular material. Arrow indicates interface between adjacent cells. Each interface typically has a junctional complex. Bar,1 µm. (d-f) Gap junctions between intimal, freshly isolated, and cultured endothelial cells, respectively. Bars,0.1 µm.

Resultados Esperados• Tratamiento con tripsina 0,001%

Endothelial cells grown in a monolayer on a Transwell filter (P990 STD glut-17), processed to epoxy resin and ultrathin sectioned to 70nm thickness.

Resultados Esperados• Tratamiento tripsina 1%

Freshly isolated and purified endothelial cell fraction. A Low magnification transmission electron micrograph (TEM) of a purified endothelial cell fraction. x 2,500.