Trabajo Histología

8/16/2019 Trabajo Histología

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“AÑO DE LA CONSOLIDACIÓN DEL MAR DE GRAU”

Universidad Nacional de Truillo

Facultad De Medicina Escuela De Medicina

Escuela académico profesional de MedicinaDepartamento de Morfología

SECCIÓN DE HISTOO!"#

TEM#$ “ASPECTOS COGNITIVOS EN EL CURSO DE HISTOLOGÍA”

#%MNOS: - DÍAZ MUÑOZ, FANNY LIZBETH- COSTTA SALDAÑA, RENATO FERNANDO- ESPEJO MENDOZA, INGRID LISSETH- ESPINOLA RIVERA, CYNTHIA KATHERINE- GRADOS ARANDA, GEORDI MAIKEL

!&%'O$ N !"

#(O DE EST%DIOS$ " AÑO ACAD#MICO

FECH#$ "$ %& M'()* %&+ "!

DOCENTE$

MC. /SCAR ALÍ FLORES ARMAS

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#S'ECTO CO!NITI)O

/1 Definir conceptos 23sicos relacionados a las técnicas 4istol5gicas1

/1 FI*#CIÓN$ E0 &+ 1(23&( 1'0* 1'(' +' 1(&1'('4256 %& 76 8&92%* 7 5('6*, 1'('4*60&(;'( 07 &08(7487(' 2&(& '+ 8('8'32&68* %&+ 8&92%* 4*6

0708'642'0 ?7@324'0 ?7& 6* 0*+* (&8'(%'6 +'0 '+8&('42*6&0 @0824'0 1*08&(2*(&0' +' 37&(8& 026* 8'326 4*60&(;' 07 4*6>27('4256 6*(3'+ 26' '48' 4*3* 76 0*1*(8& >@024* %& +' 37&08(', 1&(3282&6%*?7& 0& 17&%' 4*(8'( &6 0&442*6&0 >26'0 026 %&>*(3'( 07 &08(7487(' 4&+7+'( 07'(?728&487('.


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-1 'recisar las diferencias entre l3mina 4istol5gica< preparado 4istol5gico =o2tenci5n de la muestra1

61 Indicar secuencialmente los pasos a seguir para la o2tenci5n de una l3mina

4istol5gica1

C&ITE&IO /$

T#CNICA HISTOL/GICA

'&OCEDIMIENTOS MEDI#TOS O 'OST)IT#ES T2&6&6 1*( >26'+2%'% 1(&1'('( 4+7+'0, 8&92%*0 5('6*0 1(*4&%&68&0 %& 0&(&0 &6 +*0?7& +*0 1(*4&0*0 ;28'+&0 0& '6 %&8&62%* &0 6&4&0'(2* 4*60&(;'( +' &08(7487(' ?7&8&6@'6 &6 ;2%'. D& 3'6&(' &6&('+, 1'(' '+4'6)'( &08& *9&82;* &0 6&4&0'(2* (&'+2)'(

+*0 0272&68&0 1'0*0:a> Toma de la muestra

E208&6 %2;&(0*0 1(*4&%232&68*0 ?7& 1&(328&6 *8&6&( 37&08('0 %& 8&92%*0 5('6*01'(' 4*60&72( 1(&1'('42*6&0 208*+524'0 %& 7&6' 4'+2%'%.- M&%2'68& +' 2*102': Fi?aci5n1E0 76 1(*4&%232&68* 47' >26'+2%'%, '+ '1+24'(+* &0 %&8&6&( +' ;2%' %& +'0 4+7+'0 &231&%2( +'0 3*%2>24'42*6&0 1*08 3*(8&6 ?7& 17&%' 07>(2( +' 4+7+' *+524' %& 4+7+'0 8&92%*0 026 ?7&*47(('6 4'32*0 6*8'+&0 &6 &++*0.E08* 0& 4*6027& 263*;2+2)'6%* &48' 1*( 3&%2'68&'&68&0 ?7@324*0 %&6*326'%*0 >29'%*(&0. c> Inclusi5n

L*0 8&92%*0 >29'%*0 '%?72&(&6 42&(8' 4*60208&642' %7(&)', 1&(* 6* +' 07>242&68& 1'('?7&, %& &++*0, 0& *8&6'6 0&442*6&0 %&+'%'0. E08'0 0&442*6&0, %&+ *(%&6 %&

'+76'0 32+023'0 %& 32+@3&8(* 2+8(&6 4*6 0708'642'0 %&6*326'%'0 “%& 264+70256” '%?72&('6 8'+ %7(&)' ?7&0*3&82%*0 '+ >2+* %& 76' 6';'9' 1(*%7)4'6 0&442*6&0, 4*(8&0 * +326'0 073'3&68&%&+'%*0 8('601'(&68&0.L'0 0708'642'0 %& 264+70256 82&6&6 +' 1(*12&%'% %& 264*(1*('(0& & 26>2+8('(0& '+ 268&(2*( %& +'0 4+7+'0 8&92%*0 4*6 +' >26'+2%'% %& 0&(;2(+&0 %& 0*1*(8&. A0@ +*0 8&92%*0 +'0708'642' %& 264+70256 >*(3'6 76 +*?7& *3*6&* &6 %7(&)' 4*60208&642', '

1&0'( ?7& 070 4*31*6&68&0 87;2&(*6 *(226'+3&68& %208268' %7(&)'.


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1'(' ?7& +*0 8&92%*0 17&%'6 0&( 264+72%*0 &6 1'('>26' 0& (&?72&(& %&02%('8'(+*0 &26>2+8('(+*0 4*6 &+ 0*+;&68& %& +' 0708'642' %& 264+70256. L*0 1'0*0 ' 0&72( 1'(' +'264+70256 %& +'0 37&08('0 &6 1'('>26' 0*6:

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

= D2'>'62)'4256. L'0 37&08('0 %&02%('8'%'0 0& &647&68('6 8*8'+3&68&&3&2%'0 &6 '+4**+ &8@+24* '0*+78*Q 1&(* +' 1'('>26' 8'31*4* &0 0*+7+& &6'+4**+ 1*( +* ?7& &0 6&4&0'(2* (&&31+')'(+* 1*( 0708'642'0 ?7& 0&'64'1'4&0, 0237+86&'3&68&, %& 3&)4+'(0& 4*6 &+ '+4**+ %20*+;&( +' 1'('>26'.E08'0 0& %&6*326'6 +@?72%*0 %2'>'62)'%*(&0 * 268&(3&%2'(2*0. E9&31+*0: 2+*+,8*+7&6*, &64&6*, &+ 4+*(*>*(3*.

4= 4= I64+70256 >*(3'4256 %&+ +*?7& %& 1'('>26'. L'0 1'('>26'0 0*62%(*4'(7(*0 0'87('%*0 1(*;&62&68&0 %& +' %&082+'4256 %&+ 1&8(5+&*.

G&6&('+3&68& 0*6 0708'642'0 05+2%'0 ' 8&31&('87(' '32&68&. E208&6%2>&(&68&0 821*0 %& 1'('>26' ?7& 0& 4'('48&(2)'6 1*( 070 1768*0 %& >70256.E08*0 *042+'6 &68(& !* !* C. L' 1'('>26' 2&(;& ' !!* C. &328& ;'1*(&0?7& 0*6 37 26>+'3'+&0

d> Microtomía @o2tenci5n de los cortes> E6 &08' &8'1', +*0 8&92%*0 +' 1'('>26' 268&('6 76 0*+* +*?7& ?7&, 1*0&& +' %7(&)' +' 4*60208&642' 07>242&68&0 1'(' *8&6&( 0&442*6&0 %&+'%'0 8('601'(&68&0.L'0 0&442*6&0 %&+'%'0 * “4*(8&0” 0& *82&6&6 782+2)'6%* 2608(73&68*0 3&4624*0%20&'%*0 1'(' ?7& &6 >*(3' 30 * 3&6*0 '78*3824', 0&442*6&6 &+ +*?7& %&

1'('>26' &6 4*(8&0 %&+'%*0 %& (*0*( 762>*(3&. L*0 2608(73&68*0 0& %&6*326'6“324(*8*3*0”. e> Coloraci5n o tinci5nL*0 4*(8&0 %& +*0 8&92%*0 '%&(2%*0 ' +*0 1*(8'*9&8*0 &086 +208*0 1'(' 0&( 4*+*(&'%*0. E+ 1(*4&%232&68* %& 4*+*('4256 * 8264256 4*60208& &6 ?7& 76' &08(7487('4&+7+'( * 8207+'( '%?72&(& &01&4@>24'3&68& 76 4*+*( '9* +' '44256 %& 76' 0708'642'4*+*('68&. S& 4*602%&(' ?7& 76' &08(7487(' 0& ' 4*+*(&'%* * 8&2%* 47'6%* '+ 0&( +';'%' 4*6 &+ +@?72%* ?7& %207&+;& '+ 4*+*('68&, 6* 0& %&4*+*('. E+ 1(*1*(42*6'( 4*+*( ' +'0 &08(7487('0 ?7& 4*608287&6 76 8&92%* * 76 5('6* 0& '4& 4*6 +' >26'+2%'% %&

%2082672(+'0 &68(& 0@ >'42+28'( 07 *0&(;'4256. f> Monta?e


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iopsia$ 4*60208& &6 8*3'( 76 8(*)* %& 8&92%* %& 76 0&( ;2;*.E08& 1(*4&%232&68* %& 70* >(&47&68& &6 3&%2426' (&?72&(& &+ 3203*472%'%* %& '0&102', '6820&102', &84., ?7& 0& 782+2)' &6 8*%* '48* ?72((24*3&6*( * 3'*(.Necropsia$ &0 &+ 1(*4&%232&68* &6 &+ 47'+ 0& &8('& 3'8&(2'+%& 76 4'%;&(

#utopsia$ 4*60208& &6 &+ &087%2* '6'+@824* 0208&3824* 4*31+&8*,3'4(*045124* 324(*045124* %& +*0 5('6*0, '1'('8*0 0208&3'0 %&76 4'%;&(. S& (&'+2)' 1'(' &08'+&4&( 4'70'0 %& 37&(8&.

-1 Fi?aci5n$ 76' ;&) *8&62%* &+ 3'8&(2'+ ?7& 0& %&0&' &087%2'( 1*( 47'+?72&('

%& +*0 1(*4&%232&68*0 %&04(218*0 0& 1(*4&%& ' 07 >29'4256 4*6 +* ?7& 0& &;28'+' %&08(744256 * +2020 4&+7+'(. E0 76 1(*4&0* >@024*-?7@324* 4*31+&9* 1*( &+ 47'+0& 3'682&6& ' +'0 &08(7487('0 *(624'0 &6 &+ &08'%* 30 1'(&42%* '+ ?7&1*0&@'6 &6 ;2%'.

61 Des4idrataci5n$ 0& 4*+*4' +' 37&08(' &6 '+4**+&0 %& 4*64&68('42564(&42&68& 1'(' &+2326'( &+ '7' ?7& 4*68&6' ' ?7& +' 1'('>26' 6* &0 32042+&4*6 '7'. S& 782+2)' &8'6*+ !, !, $ !!.

71 #claraci5n$ 0*6 0*+;&68&0 ?7& 1(*%74&6 8('601'(&642' &6 +*0 8&92%*0, '%&30%& 0*+72+2)'( +' 1'('>26'. E68(& &++*0 0& &647&68('6. 2+*+. 8*+7*+. '4&8*6',&64&6*. E+ 30 70'%* &0 &+ 2+*+.

81 Inclusi5n en parafina$ +' 1'('>26' &0 76' 3&)4+' %& 2%(*4'(7(*0 0'87('%*0?7& 82&6&6 %2>&(&68&0 1768*0 %& >70256. P'('>26' +'6%' >76%& ' C +'1'('>26' %7(' ' C. LA 1'('>26' 0& 1*6& &6 &087>' %& 47+82;* ' 76*0('%*0 1*( &6423' %& 07 1768* %& >70256.S& 073&(& +' 37&08(', 0& ++&;' ' &087>' 76*0 32678*0 1'(' ?7& +' 1'('>26'1&6&8(& &6 +*0 8&92%*0. L7&* 0& &6>(@' (704'3&68& 4*+*4'6%* &+ (&4212&68& &62&+*.

01 'reparaci5n del taco$ 0& 4*(8' 76 8(*)* %& 1'('>26' ?7&4*682&6& +' 37&08(' &6 >*(3' %& 12(32%& 8(764'%', %& 3'6&(' 8'+

?7& +' 3203' ?7&%& 0*(& +' '0& 3&6*(. S& 1&' +' '0& 3'*(@' 76 8'?728*%& 3'%&(' ?7& 0&(;2( 1'(' 0*08&6&(+* &6 &+ 324(58*3*.


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91 Corte$ 0& (&'+2)' 3&%2'68& &+ 70* %&+ 324(58*3*. E+ 324(58*3*%& 4*6&+'4256 1&(328& 4*(8'( &+ 8&92%* %&0170 %& >29'(+* 1*( >(@*,4*6&+' 4*6 %252%* %& 4'(*6* +* &6%7(&4& 1'(' ?7& +7&* 17&%' 0&( 4*(8'%*.E0 76 38*%* (12%* 1*( &0* 0& 70' 1'(' &+ %2'650824* %& 2*102'0 *8&62%'0&6 &+ '48* ?72((24* 1'(' ?7& &+ 42(79'6* 1(*4&%' 0&6 &08& 26>*(3&.

:1 Colocaci5n so2re portao2?eto$ &>&487'%*0 +*0 4*(8&0 0& 4*+*4'6 &6

'7' 822' 1'(' ?7& 0& &82&6%'6 +7&* 0& (&4*&6 4*6 76 1264&+ 0& +*0&82&6%&6 &6 &+ 1*(8'*9&8*0 0& %&9'6 0&4'( 1'(' 07 1*08&(2*( 4*+*('4256.;1 DesparafiniBaci5n$ 0& %&& &8('&( 8*%* +' 1'('>26' %&+ 8&92%*

'4+'('(+* 67&;'3&68&. S& 782+2)' 2+*+./.1 Hidrataci5n$ 0& %&& 2%('8'( &+ 8&92%* ' ?7& +' 3'*(@' %& +*0 4*+*('68&0

0*6 %& '0& '47*0'. P'(' &++* 0& 4*+*4' &+ 1*(8'*9&8* &6 0*+742*6&0 %& &8'6*+%& 4*64&68('42*6&0 %&4(&42&68&0. E8'6*+ !!, $, !, ! A7' %&082+'%'.

//1 Coloraci5n$ &0 76 4*31+&8* 4*31+&9* >@024*?7@324* ?7& +& 4*6>2&(& 4*+*( ' +*08&92%*0 %7('68& 82&31*0 1(*+*6'%*0. L'0 3*+47+'0 %& 4*+*('68&0 82&6&6 76

(71* ?7& &0 &+ ?7& +& 4*6>2&(& &+ 4*+*(: 4(*35>*(* *8(* ?7& +* >29' '7*4(*3*./-1Des4idrataci5n$ 1'(' 1(&1'('( +*0 1(&1'('%*0 1*( 82&31* 1(*+*6'%*.

S& 073&(& &+ 1(&1'('%* &6 0*+742*6&0 %& &8'6*+ %& ('%7'4256 4(&42&68&0


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