Proyecto Bq

7/24/2019 Proyecto Bq

1/18

ndice

Introduccin----------------------------------------------------------------------

UNIVERSIDAD NACIONAL AUTNOMA DE MXICOFACULTAD DE ESTUDIOS SUPERIORES CUAUTITLN

DEPARTAMENTO DE CIENCIAS BIOLGICAS

SECCIN DE BIOQMICA Y FARMACOLOGA HUMANA

BIOQUIMICA DE SISTEMAS

Protocolo de investigacin

Efectos bioqumicos presentes porla accin de un hepatoprotectorCholal frente la Hepatoxixidad del

paracetamol en ratas wistar

ic! "armacia

#ntegrantes

*Arroyo Ibarra Viridiana

* Lpez Arratia Berenice

*Rayn Rodrguez Carmen Jacqueline

*Treo !"#imo $d%on Alon%o

$rupo% &'(&

)emestre% *(&+,#


2/18

Marco terico---------------------------------------------------------------------

Objetivos ---------------------------------------------------------------------------

Hiptesis------------------------------------------------------------------------------

Material---------------------------------------------------------------------------------

Metodologa---------------------------------------------------------------------------

Cotizacin -----------------------------------------------------------------------------

Cronograma----------------------------------------------------------------------------

Referencias -----------------------------------------------------------------------------

I!RO"#CCI$%

Los te!"os #e$%t!&os 'o so(o so' (os te!"os )!&os e' )e&e$to)es *+),+&o(-.!&os (os /0e t!e'e' (+s,%s *0e)tes &o'&e't)+&!o'es "e ,e"!&+,e'tos1 S!'o +/0e((os e' (os /0e se e*e&t2+' ,%s s0,et+3o(!s,o4 s0 +(,+&e'+,!e'to o s0 e(!,!'+&!-'1 E( #5.+"o4 .)+&!+s + s0 (o&+(!6+&!-' +'+t-,!&+4 +s0 .)+' !))!.+&!-' s+'.05'e+ 7 +( $+$e( /0e 0e.+ e( ,et+3o(!s,o 7 e8&)e&!-' "e (os ,e"!&+,e'tos$0e"e &o'&e't)+) +(.0'os "e e((os + &o'&e't)+&!o'es e't)e "!e6 7 &!e' 9e&es s0$e)!o)es + (os "e (+


3/18

s+'.)e o ,0s&0(o1 E( )!es.o "e +$+)!&!-' "e e*e&tos #e$+toto8!&os e' e( #o,3)e4 0'o "e (os ,%s*)e&0e'tes "es"e e( $0'to "e 9!st+ est+"5st!&o se "e3e e' 30e'+ ,e"!"+ + este *e'-,e'o1:C+s&+(es;?@

L+ $)ese'&!+ "e (os ,e"!&+,e'tos e' e( #5.+"o $0e"e te'e) &o'se&0e'&!+s 9+)!+"+s se.2' (+"!)e&&!-' "es"e (+ /0e se +'+(!&e (+s $os!3(es !'te)+&&!o'es1 Es $)e&!so )e&o)"+)4 e' $)!,e) (0.+)4/0e e( #5.+"o es e( )es$o's+3(e $)!'&!$+( "e( ,et+3o(!s,o "e (os ,e"!&+,e'tos1 Los #e$+to&!tos"!s$o'e'4 e' e*e&to4 "e 0' e/0!$o e'6!,%t!&o 7 "!*e)e'&!+"o4 &+$+6 "e ,o"!*!&+) s0 est)0&t0)+ e'

.e'e)+( #+&!+ 0'+ !'+&t!9+&!-'4 $e)o t+,3!' ,e"!+'te (+ $ote'&!+&!-' "e s0s $)o$!e"+"es*+),+&o(-.!&+s1 Est+ 3!ot)+'s*o),+&!o' #e$%t!&+s so' es$e&5*!&+s 7 est%' )e.0(+"+s4 es "e&!)4 so's0s&e$t!3(es "e +&e(e)+&!-' o "e "es+&e(e)+&!-'1 S!e,$)e !,$o)t+'te4 (+ !'te)9e'&!-' ,et+3-(!&+ "ete!"o #e$%t!&o &o'"!&!o'+ (+ ,+s+ "e $)!'&!$!os +&t!9os &+$+&es "e +&t0+) so3)e (os )e&e$to)est!s0(+)es 74 $o) e((o4 so3)e s0s e*e&tos *+),+&o(-.!&os1 Se $)o"0&e' ot)os ,e&+'!s,os s! se t!e'e e'&0e't+ (+ )e(+&!-' ,e"!&+,e'to;#e$+to&!to1 E' este &+so4 e( +.e'te te)+$0t!&o4 o ,%s )+)+,e'te 0'$)o"0&to "e 3!ot)+'s*o),+&!o' "e( ,!s,o4 $0e"e ee)&e) so3)e e( te!"o #e$%t!&o e*e&tos t-8!&os$)o"0&!e'"o 0'+ +(te)+&!-' "e (+s est)0&t0)+s &e(0(+)es o "e (+ &+$+&!"+" *0'&!o'+( "e( te!"o:C+s&+(es; ?@

E( "+o +( #5.+"o4 t+,3!' &o'o&!"o &o,o #e$+toto8!&!"+"4 #+&e /0e este -).+'o *0'"+,e't+

*0'&!o'e !'+"e&0+"+,e'te o "e *o),+ !))e.0(+)1 E( #5.+"o es e( ,+7o) -).+'o "e( &0e)$o 7 t!e'e,0+s *0'&!o'es 9!t+(es1 L+ t+)e+ ,%s !,$o)t+'te "e( #5.+"o es *!(t)+) s0st+'&!+s t-8!&+s "e s0&0e)$o4 &o,o e( +(&o#o( 7 ,0os ,e"!&+,e'tos "!*e)e'tes4 &o,o (os *%),+&os "e /0!,!ote)+$!+4(os +'t!3!-t!&os1 Ot)+s *0'&!o'es !,$o)t+'tes /0e )e+(!6+ e( #5.+"o so' Co(+3o)+ &o' e( 3+6o $+)+)et!)+) "e (+ s+'.)e .(-30(os )oos 9!eos o "++"os1 P)o"0&e (+ 3!(!s4 0'+ s0st+'&!+ /0e se (!3e)+ +(!'test!'o $+)+ +70"+) e' (+ +3so)&!-' 7 "!.est!-' "e (+s .)+s+s 7 $)o$o)&!o'+ 0' ,o"o $o) e( /0e e(#5.+"o $0e"e e8&)et+) $)o"0&tos "e "eseo P)o"0&e *+&to)es "e &o+.0(+&!-' /0e so'*0'"+,e't+(es $+)+ *o),+) 0' &o%.0(o /0e "ete'.+ e( s+'.)+"o P)o&es+ 7 &o'se)9+ (+s 9!t+,!'+s4(os ,!'e)+(es4 (+s $)ote5'+s4 (+s .)+s+s 7 (+ .(0&os+ :+62&+)@ "e (+ "!et+1

P+)+&et+,o( Es 0'o "e (os ,e"!&+,e'tos ,%s 0s+"os $o) s0s $)o$!e"+"es +'+(.s!&+s 7+'t!t),!&+s1 Des"e #+&e ,0os +os se &o'o&e s0 &+$+&!"+" "e $)o9o&+) "+o #e$%t!&o +.0"o&o' 'e&)os!s e' (+ 6o'+ "e( +&!'o #e$%t!&o Este "+o es "e t!$o !'t)5'se&o 7 se $)o"0&e s!e,$)e +(!'.e)!) ,%s "e >;


4/18

C+s&+(es4 M1 Fe))+'"!64 F1 :?@ As$e&tos 3!o/05&os 7 *+),+&o(-.!&os e' "!s*0'&!o'es #e$%t!&+s1M+")!"4 &o'seo s0$e)!o) "e !'9est!.+&!o'es &!e't5*!&+s

!ARC& T$&RIC&

He$+to$)ote&to) So' +/0e((os *%),+&os /0e ,eo)+' e( *0'&!o'+(!s,o "e (+ &(0(+ #e$%t!&+$e),!t!e'"o /0e $0e"+' 3(o/0e+) +(.0'os +.e'tes #e$+tot-8!&os4 +(.0'os so' +,!'o%&!"os &o,o (+,et!o'!'+ 7 ot)os &o,o e( %&!"o t!o&t!&o1

Los #e$+to$)ote&to)es est!,0(+' (os $)o&esos "e "eto8!*!&+&!-' #e$%t!&+4 +&t0+'"o &o,o#e$+to$)ote&to)es 7 #e$+to)e.e'e)+"o)es4 "e3!"o + /0e ,+'t!e'e' (+ !'te.)!"+" "e (+s ,e,3)+'+s&e(0(+)es "e( #e$+to&!to +0,e't+' (os '!9e(es #e$%t!&os "e %&!"o +s&-)3!&o !'#!3e' (+ o8!"+&!-'NADPH +0,e't+'"o (+ NADPH;&!to&)o,o & )e"0&t+s+ !'#!3e' (+ $e)o8!"+&!-' (!$5"!&+ + '!9e,!&)oso,+( #e$%t!&o 7 (+ *o),+&!-' "e ,+(o'"!+("e#!"o #e$%t!&o "!s,!'07e' (+ $)o"0&&!-' "e,o'-8!"o "e &+)3o'o + '!9e( #e$%t!&o !'"0&e' e( s!ste,+ e'6!,%t!&o ,!&)oso,+( #e$%t!&o &!to&)o,oP; $)e9!e'e' (+ &o(est+s!s !'"0&!"+ $o) s0st+'&!+s t-8!&+s4 +0,e't+'"o e( *(0o 3!(!+) 7 (+e8&)e&!-' "e s0st+'&!+s t-8!&+s $o) 95+ 3!(!+)1 D!s,!'07e' (os '!9e(es $(+s,%t!&os "e t)+'s+,!'+s+s 7$)o,0e9e' (+ )e&0$e)+&!-' "e (+s *0'&!o'es #e$%t!&+s4 (o /0e !'"!&+ 0'+ )e"0&&!-' "e( "+o#e$+to&e(0(+) !'"0&e' (+ *o),+&!-' "e( ,RNA $+)+ e( *+&to) "e &)e&!,!e'to "e( He$+to&!to :FCH@4

/0e &o't)!307e + s0 )e.e'e)+&!-' est!,0(+' (+ $)o(!*e)+&!-' "e( )et5&0(o e'"o$(%s,!&o (!so "e(#e$+to&!to 7 +&e(e)+ (+ $)o(!*e)+&!-' "e #e$+to&!tos +0,e't+' (+ +&t!9!"+" "e (+ o)'!t!' "e&+)3o8!(+s+7 e( 5'"!&e ,!t-t!&o +0,e't+' (os '!9e(es "e .(0t+t!o' ,!to&o'")!+( #e$%t!&o 7 (+ +&t!9!"+" "e (+.(0t+t!o' )e"0&t+s+ ,!to&o'")!+( 7 "e (+ .(0&os+;J;*os*+to #e$%t!&+s4 ,eo)+'"o (+ &+$+&!"+" "e)e.e'e)+&!-' "e( .(0t+t!o' )e"0&!"o 7 (+ s5'tes!s "e .(0&-.e'o #e$%t!&o1

Me&+'!s,os "e +&&!-'1

Los ,e&+'!s,os "e +&&!-' "e (os #e$+to$)ote&to)es &o's!ste'

K Mo"!*!&+&!-' "e (+ ,e,3)+'+ &e(0(+) e8te)'+ "e (os #e$+to&!tos !,$!"!e'"o e( !'.)eso "e(+s to8!'+s +( !'te)!o) "e (+s &(0(+s

K Est!,0(+&!-' "e (+ +&t!9!"+" "e (+ $o(!,e)+s+ A '0&(eo(+)4 +0,e't+'"o (+ s5'tes!s )!3oso,+"e $)ote5'+s 7 (+ &+$+&!"+" "e )e.e'e)+&!-' "e( #5.+"o 7 (+ *o),+&!-' "e '0e9os#e$+to&!tos

K U' e*e&to +'t!o8!"+'te /0e &o't)+))est+ (+ +&&!-' "e (os )+"!&+(es (!3)es /0e se *o),+'&o,o ,et+3o(!tos )e+&t!9os $o) +&&!-' "e (+s to8!'+s 7 /0e "++' (+s ,e,3)+'+s&e(0(+)es1

P)0e3+s 3!o/05,!&+s

K Co(este)o(K A(30,!'+K P)ote5'+s tot+(es

&&'I(I( ") *R#)+&( +IO,#MIC&(

L+s $)0e3+s &o' (+s /0e $o"e,os $+)+ "!+.'ost!&+) 7 9!.!(+) e'*e),e"+" #e$%t!&+4 e' este &+so (+#e$+toto8!&!"+" !'"0&!"+ $o) e( A&et+,!'o*' so' (+s s!.0!e'tes1


5/18

+ilirrubina total bilirrubina directa.L+ 3!(!))03!'+ es 0' "e)!9+"o "e (+ "e.)+"+&!-' "e (os

.(-30(os )oos1 Po) (o .e'e)+(4 $+s+ + t)+9s "e( #5.+"o 7 es e(!,!'+"+ "e( o).+'!s,o1 Pe)o s!esto 'o o&0))e4 + &+0s+ "e 0'+ e'*e),e"+" #e$%t!&+4 (os '!9e(es "e 3!(!))03!'+ e' (+ s+'.)es0e(e' +0,e't+) 7 (+ $!e( se to)'+ +,+)!((e't+ :!&te)!&!+@1 Los +'%(!s!s "e 3!(!))03!'+ $0e"e' se)tot+( :,!"e' e( '!9e( "e to"+ (+ 3!(!))03!'+ e' s+'.)e@ o "!)e&t+ :,!"e' so(+,e'te (+ 3!(!))03!'+/0e #+ s!"o $)o&es+"+ $o) e( #5.+"o 7 &o,o )es0(t+"o se #+ 0'!"o + ot)+s s0st+'&!+s/05,!&+s@1

Te'!e'"o &o,o 9+(o) 'o),+( (+ &o'&e't)+&!-' ; 1J ,. "L es$e)+'"o 0' +0,e'to e' estos9+(o)es +( !'"0&!) e( e*e&to #e$+tot-8!&o

&lb/mina protena total.L+ $)ote5'+ es 'e&es+)!+ $+)+ *o),+) 7 ,+'te'e) (os ,2s&0(os4

(os #0esos4 (+ s+'.)e 7 e( te!"o "e (os -).+'os1 A 9e&es4 &0+'"o #+7 0' $)o3(e,+ &o' e(#5.+"o4 ste 'o $0e"e $)o"0&!) $)ote5'+s 74 $o) (o t+'to4 (os '!9e(es "e $)ote5'+s "!s,!'07e'1Los +'%(!s!s "e (+s *0'&!o'es #e$%t!&+s ,!"e' es$e&5*!&+,e'te (+ +(32,!'+ :(+ $)ote5'+ ,%s!,$o)t+'te "e (+ s+'.)e $)o"0&!"+ $o) e( #5.+"o@ 7 (+ &+'t!"+" tot+( "e to"o t!$o "e $)ote5'+s

e' (+ s+'.)e1

Te'!e'"o &o,o 9+(o) 'o),+( $+)+ $)ote5'+s tot+(es (+ &o'&e't)+&!-' J1; >1J ,. ,L 7 $+)+ (++(32,!'+ 1; 1= ,. "L es$e)+'"o 0' +0,e'to e' estos 9+(o)es +( !'"0&!) e( e*e&to#e$+tot-8!&o1

P)0e3+ o$&!o'+(

&spartato aminotransferasa 0&(!1.Est+ e'6!,+4 /0e "ese,$e+ 0' $+$e( e' e(

$)o&es+,!e'to "e $)ote5'+s4 est% $)ese'te e' e( #5.+"o4 e( &o)+6-'4 (os ,2s&0(os 7 (os)!o'es1 C0+'"o e( #5.+"o se (es!o'+ o !'*(+,+4 (os '!9e(es "e AST e' (+ s+'.)e s0e(e' se)e(e9+"os1

Te'!e'"o &o,o 9+(o) 'o),+( $+)+ +s$+)t+to +,!'ot)+'s*e)+s+ (+ &o'&e't)+&!-' =; = UILes$e)+'"o 0' +0,e'to e' estos 9+(o)es +( !'"0&!) e( e*e&to #e$+tot-8!&o1


6/18

*erfil 2ep3tico

E( $e)*!( #e$%t!&o es 0' +'%(!s!s "e s+'.)e e' e( /0e se ,!"e (+ $)ese'&!+ "e +(.0'+s e'6!,+s4$)ote5'+s 7 3!(!))03!'+ e' s+'.)e4 &o' e( o3et!9o "e "ete),!'+) s! e8!ste +(.0'+ +(te)+&!-' e' e(#5.+"o1L+s $)0e3+s /0e $0e"e' *o),+) $+)te "e( $e)*!( #e$%t!&o so' (+s s!.0!e'tes

T+3(+


7/18

@ Ne&)os!s e' s+&+3o&+"o o 'e&)os!s e)os!9+ "est)0&&!-' "e #e$+to&!tos +!s(+"os "e (+ $(+&+(!,!t+'te $e)!$o)t+(4 &o' !'te))0$&!-' "e st+1 E' (0.+) "e (os #e$+to&!tos #+7 !'*!(t)+&!-' (!'*o&!t+)!+4/0e t!e'"e + )o"e+) #e$+to&!tos4 .e'e)+(,e'te 3+(o'!6+"os4 e' 95+s "e "es+$+)!&!-'1 No se +$)e&!+(+ et+$+ "e 'e&)o*+'e)os!s1 L+ $)ese'&!+ "e 'e&)os!s e' s+&+3o&+"o $0e"e s!.'!*!&+) e( $+so + (+((+,+"+ #e$+t!t!s &)-'!&+ +&t!9+1

T+3(+ 1 Res0(t+"os es$e)+"os $o) (ote1

; V+(o)es se ,+'t!e'e' 'o),+(es

A0,e'to "e 9+(o)es 'o),+(es

; D!s,!'0&!-' "e 9+(o)es 'o),+(es

F%),+&os + 0t!(!6+)

C#o(+( :He$+to$)ote&to)@

P)e&!o

ot

e

Cuanti-cacin de

bilirrubinas totales(, (!+. mg/ d

Protenas totales

+!0, 1!+ mg/ m

2lb3mina

0!0, .!4 mg/ d

2)5

04, 4* 6#/

& 7/, 7/, 7/, 7/,

* 7/, 7/, 7/, 7/,

0

7 , , 7

. 7/, 7/, 7/, 7/,


8/18

4orma farmac5utica formulacin C+"+ +,$o((et+ "e ,( &o't!e'e

S0(*+to "e so"!o +'#!")o 1 . S0(*+to "e ,+.'es!o +'#!")o 1> . C(o)0)o "e so"!o 1


9/18

.%st)!&+ e !'test!'+(Po) s0 $+)te e( !'os!to( s!e'"o +(&o#o( :$o(!+(&o#o( "e &+"e'+ &e))+"+@ +&t2+ e' "!9e)sos $)o&esos&o,o (!$ot)-$!&o :&o'*!),+"o $o) G+9!' 7 M& He')7@ t!e'e (+ $)o$!e"+" "e ,o9!(!6+) (+s .)+s+s 7((e9+)(+s +( to))e'te s+'.05'eo $+)+ s0 e(!,!'+&!-'E( !'os!to( t+,3!' *o),+ $+)te "e (os $)!'&!$+(es *os*o.(!&)!"os /0e +&t2+' &o,o &o,$o'e'tes "e(+s ,e,3)+'+s &e(0(+)es 7 "e (+s (!$o$)ote5'+s "e t)+'s$o)te4 "o'"e e( "!+&!(.(!&)!"o s!)9e &o,o$)e&0)so) t+'to "e (+ 3!os5'tes!s "e( *os*+t!"!(;!'os!to( &o,o "e (+ *os*+t!"!(.(!&e)!'+ 7 $0e"e se)

se$+)+"o &o,o "!&!(.(!&e)o( 7 t)!*os*+to "e !'os!to(1 Es ese'&!+( e' e( &)e&!,!e'to "e (+s &(0(+s "e (osse)es #0,+'os :E+.(e@So)3!to( A(&o#o( &o' +&t!9!"+" es$e&5*!&+ e' e( +$)o9e+,!e'to 7 +s!,!(+&!-' "e (+s 9!t+,!'+s "e(&o,$(eo B +"e,%s (+ 95+ "e( so)3!to( :$o(!o(@ es )es$o's+3(e "e (+ *o),+&!-' "e *)0&tos+ + $+)t!) "e(+ .(0&os+ e' $)ese'&!+ "e (+ e'6!,+ so)3!to( "es#!")o.e'+s+ + '!9e( #e$%t!&o $o) (o /0e t+,3!'t!e'e +&&!-' e'e).!6+'teSo)3!to( 0'to &o' e( s0(*+to "e ,+.'es!o 7 e( s0(*+to "e so"!o t!e'e' +&&!-' &o,o &+t%)t!&os:"es!'to8!&+'tes@4 7+ /0e +&t2+' )%$!"+,e'te 7 s!' e*e&tos se&0'"+)!osCHOLAL MODIFICADO ,+'t!e'e e' -$t!,+s &o'"!&!o'es e( *0'&!o'+,!e'to #e$%t!&o 0t!(!6%'"o(o&o,o *%),+&o "e $)!,e)+ e(e&&!-' 7+ /0e $o) (os &o,$o'e'tes "e s0 *-),0(+ &o))!.e e,et+3o(!s,o #e$%t!&o4 )e.0(+'"o (os '!9e(es "e &o(este)o( 7 t)!.(!&)!"os4 +s5 &o,o (+ )e(+&!-'

LDLHDL1Los t)!.(!&)!"os /0e *0'&!o'+' &o,o (5$!"os "e "e$-s!to o +(,+&e'+,!e'to so' +&t!9+,e'tes!'tet!6+"os e' (+ &(0(+ #e$%t!&+1 Estos t)!.(!&)!"os so' "es"o3(+"os e' e'e).5+ "e (+ ,!s,+,+'e)+ /0e (os $)o9e'!e'tes "e (+ "!et+ 7 e( &o(este)o( )est+'te es t)+'s$o)t+"o + (os te!"os$e)!*)!&os4 (+s (!$o$)ote5'+s t+,3!' t)+'s$o)t+' e( &o(este)o( e' "!)e&&!-' !'9e)s+ "e (os te!"os$e)!*)!&os +( #5.+"o :t)+'s$o)te !'9e)so "e( &o(este)o(@ "+'"o o)!.e' e't)e ot)+s !'te)+&&!o'es + (+!"e+ 3%s!&+ "e (+s t)es 95+s


10/18

C++ &o'


11/18

; A(&o#o(!s,o e( &o's0,o &)-'!&o "e +(&o#o( $0e"e $ote'&!+) (+ to8!&!"+" #e$%t!&+ "e( $+)+&et+,o(1De3e e9!t+)se t)+t+,!e'tos $)o(o'.+"os o + "os!s +(t+s1

; I's0*!&!e'&!+ #e$%t!&+ "+"o /0e se ,et+3o(!6+ $)!'&!$+(,e'te $o) est+ 95+4 "e3e e9!t+)se o)e"0&!)se (+ "os!s1

; A'e,!+ "e3!"o + (+ $os!3(e +$+)!&!-' "e +(te)+&!o'es s+'.05'e+s4 se )e&o,!e'"+ $)e&+0&!-' e'estos $+&!e'tes 7 e9!t+) t)+t+,!e'tos $)o(o'.+"os1

; De*!&!e'&!+ "e .(0&os+;J;*os*+to;"es#!")o.e'+s+ e( $+)+&et+,o( + "os!s +(t+s $0e"e $)e&!$!t+)+'e,!+ #e,o(5t!&+ +.0"+ e' estos $+&!e'tes1

; A(e).!+ + s+(!&!(+tos e( $+)+&et+,o( es 0'+ +(te)'+t!9+ e' $+&!e'tes +().!&os + s+(!&!(+tos 0 ot)osAINE1 S!' e,3+).o4 se #+' "+"o &+sos "e )e+&&!-' &)06+"+1

; Se #+' "es&)!to &+sos "e !'to8!&+&!-' +&&!"e't+( "e '!os $e/0eos $o) (+ $)es&)!$&!-' "e e'9+ses"e t+,+o .)+'"e /0e ((e9+' e)!'.0!((+ "os!*!&+"o)+ $o) ,(4 e' 9e6 "e e'9+ses $e/0eos &o'&0e't+.ot+s "os!*!&+"o)1 P+)+ ,!'!,!6+) este )!es.o se +&o'se+ $)es&)!3!) $+)+ '!os $e/0eos e(e'9+se &o' "os!*!&+"o) $o) &0e't+.ot+s1 Po) este ,ot!9o e' USA "es"e e( +o


12/18

!',e"!+t+,e'te "es$0s "e (+ !'.est!-'4 s!'o + $+)t!) "e( te)&e) "5+1 P0e"e $)o"0&!)se (+ ,0e)te $o)'e&)os!s #e$%t!&+1 As!,!s,o4 $0e"e +$+)e&e) *+((o )e'+( +.0"o1

L+ so3)e"os!s "e $+)+&et+,o( se e9+(2+ e' &0+t)o *+ses4 /0e &o,!e'6+' e' e( ,o,e'to "e (+!'.est!-' "e (+ so3)e"os!s1

FASE I : #o)+s@ ,eo)5+ &(5'!&+ &o,!e'6+' + e(e9+)se (os '!9e(es "e AST4 ALT4 3!(!))03!'+ 7

$)ot)o,3!'+1

FASE III :?;=J #o)+s@ $!&o "e #e$+toto8!&!"+" $0e"e' +$+)e&e) 9+(o)es "e 1 $+)+ (+ AST1

FASE IV :?;> "5+s@ )e&0$e)+&!-'1 P0e"e +$+)e&e) #e$+toto8!&!"+"1 L+ ,5'!,+ "os!s t-8!&+ es "e J .e' +"0(tos 7 ,%s "e


13/18

Co'se)9+&!-' e( 9!+( "e3e &o'se)9+)se + T +,3!e'te

O+6)!I7O%

De,ost)+) (os e*e&tos 3!o/05,!&os "e( #e$+to$)ote&to) C#o(+( e' $)ese'&!+ "e 0' #e$+toto8!&o$+)+&et+,o(4 ,e"!+'te (+ +",!'!st)+&!-' o)+( "e +,3os *%),+&os4 0t!(!6+'"o )+t+s !st+) $+)+ /0e +t)+9s "e +(.0'+s $)0e3+s 3!o/05,!&+s se e9!"e'&!e' (os &+,3!os ,et+3-(!&os e*e&t0+"os $o) (+s

+(te)+&!o'es !'"0&!"+s1

HI*O!)(I(%

S!4 se +",!'!st)+ e' )+t+s !st+) $+)+&et+,o( &o,o 0' #e$+tot-8!&o .e'e)+)% "!s*0'&!o'es #e$%t!&+s"e( #5.+"o e9!"e'&!%'"ose e' (+ "!s,!'0&!-' "e $)ote5'+s tot+(es 7 3!(!))03!'+4 e'to'&es +(+",!'!st)+) C#o(+( &o,o #e$+to$)ote&to) se 9e)% 0'+ )e.0(+&!-' "e (+s *0'&!o'es "e( #5.+"o"e9o(9!e'"o (+ est+3!(!"+" + (os 9+(o)es 'o),+(es "e $)ote5'+s1

M&!)RI&'


14/18

M)!O"O'O8I&

Re%iduo 'i%po%icin8eringa 9asura municipal

$uantes : cubre boca 9asura municipal

5ubo Colocar en el contenedor indicadoCad;veres de animales Envolver en papel reciclado : bolsa de pl;stico< colocar en lugar

indicado)ondas orales 9asura municipalCama sanitaria Colocar en bolsa : despu=s en la basura municipal

2gu>as Colocar en el contenedor ro>o< rgido de ?P9#@s

$(ecto )epatoprotector del )epap)il enrata% i%tar inducida% a e(ecto

Realizar prueba%clnica% y necrop%ia

Realizar tratamiento% de acuerdo al

'ieta normal durante todo el'ieta normaldurante todo

el proyecto,no %e leadmini%trar"nada or %er

'ieta normaldurante todo el

proyecto

Lote -

+aracetamol

Lote .

+aracetamol /

Lote 0

Control

Lote 1

C)olal

!arcar, %e#ar, pe%ar y di%tribuir a la%rata% de acuerdo al modelo CCJ en . lote%


15/18

CO!I9&CI$%

Dos!s t-8!&+ $+)+ !'"0&!) e( e*e&to #e$+tot-8!&o &o' $+)+&et+,o(


16/18

C?ABA$?22

as de

: *

loteos

(+,octote &%2limentacinnormalote *%2dministracin vaoral de cholal

ote 0%alimentacinnormalote .%2dministracin deparacetamol :cholal

(,octote &%2limentacinnormalote *%2dministracin vaoral de cholal


(1,octote &%2limentacinnormalote *%2dministracin vaoral de cholal


(4,octote &%2limentacinnormalote *%2dministracin vaoral de cholal


&(,octote &%2limentacinnormalote *%2dministracin vaoral de cholal


o2lnoo2dor

oalnoo2dpach

as de

: *

el

.


17/18

R$5$R$2CIA6

C2?K< 8!% 9ioqumica Experimental< &a Ed< Editorial 2cribia< LaragoIa< &4++!

CAM$#< ? : P2?EE< 2% 5=cnicas de #nvestigacin 9ioqumica< &a Ed< Editorial 2lhambra< adrid< &4+.!

)5?NE?< % 9ioqumica< *a Ed< Editorial ?evert=< 9arcelona< &410!

c$#DE?N< $% 9ioqumica 2plicaciones Clnicas< 0a Edicin< Editorial c$raw Hill< =xico< &41+

Limmerman H8! 5he adverse eOects of drugs and other chemicals on the liver! *nd ed! Philadelphia% ippincottMilliams MilQinsJ &444!

"arrel $C! Hepatopata causada por f;rmacos< anest=sicos : toxinas! En% )leisenger : "ordtran eds!

Enfermedades gastrointestinales : hep;ticas% "isiopatologa diagnstico : tratamiento! a ed! 9uenos 2ires%Ed! PanamericanaJ *((.! p! &.1+,&'0!


18/18

Proyecto Bq

Documents

Transcript of Proyecto Bq