SC10 R.epo :It{.~:~naZ21λ.vaU111γ. ~\/ 01. 36‘No. 1 pp. 1-]ヲJ uly 1991

Studies ¥UiH th~ CtmdiH了'fill対日日杷(lfthe

Gametophyie jJ] NlmH，;i:

(l9) Rehuionships hetween the Stωn a:nd Seta hl SOIue 。fBryaceae句 BartJramiIaceae ~mI Dl.cranarea，丘

I;mwo KA九iVAI

広三2naZatV{l

Abstrad: Some paraHeEsm in inn色rstructures is found in both the stem emd seta. But th己reis also variatii)D in pattξ111S of organization of conducting tissues in gamεtophyt己品ndsporophyt日 ofn10sses

ln Polyb允humcommune HEDVV-.， the ouler-most layer of seta and the leaf of ganぼphyte， th0 n日xtinner layer of seta and epid告rmisof stern aI日 respectively.st.ained sirnj]ar colors. And th色 ou同士 mosllayεrof sporophシteand the Jeaf initial of gamelophy日 maybε founc1 3. sirniJ ar division patt己rnin the early stage. Ii both diffeγ己ntiョtionpatt日rnanc1 propel'ties ShO¥Ning simi1ar staining are considered in POかirichurn，th宕 outer-mostlayer of the seta may be a 1己af-Iikeorganization

1. Kli1tnxi!ud;lon

and hav行 beenshown to be homology

ZU¥!lMERlv'IA1¥) BOLD INOUE 1978. While other reports

maintained neither botI1 ~~ iLU to thεantithetic

BOWER CANIPBELL NISHIDA 1980.

H in both stem and seta are taken into a number of distincl

combinations are found. Some parallelism in structurεIηay exist betweεn the two のnera-

tions. ， there is great variation the of tissues in

and of mosses

In this pap巳r，anatomical characteristics of seta are compared with thosεof thεstem，

and the rεlationshiDs betvveen the stem and seta are discussed.

2， Mat四 ialsanrllll!!dhm:ls

2. 1 Mat配rians

Fresh materials of and are obtained from the Botanical 'Garden

material~; used for this are specimens oI mosses from

AlI are in the lYIoss Herbarium of Kanazawa

Polytrichaceae: 幹rumHEDW.， commune HEDViん

HEDWパ

2 KAWAI

lltrichum u何dulatum(HEDVV，) P. BEAUV

dichョtomum

(HEDVv.) 孔 "，v{"vnu"，vi'lC1rYVJ UJ.tnUA，iUI:lIl?n KINDBけBESCH.ε lanceolatum HOOK. f. et

turbiηatun1

et

均

}コ'ohlialum 戸ulchnum〆 Pohlia

nuians LINDB.， Acidodontium exaltcdum (NIITT.)

Mniaceae: Mηzum rmcγU!i.，r¥/I/u:m Doz. et Mηium slriatu1付加lITT.

Bartramiaceae: Bartramia HED'iN.， Philonotis MITT.， Philonotis

thwaitesii MITT。

Dicranacεae: Dicranum

BESC!L Dicranum

]AEG.，

20 2 M:etnlO(ls

MITT.， Dicraηum jeψonicum Dicra幻um

jJicranum virideで人加.khodense

BESC日.

Observation of inner structures of stern and s己ta

For anatomical mIc1'otome sectio口Sof the rnosses are

+:hεfucsin acid method.

'Nith diffεrent co10'1'8

Sections of the seta of commune are treated 'with 3 oI green

solution AJter 2 the section.s己retreated with one dnコ of合osin

solutlon After 2 houγs， the sections are '¥'I7ashed with vvater and observed.

Sections of the stem ofιhum commune are treated v¥l1th 2 of green

solutioョ andaft町 50 3 of日oSlnsolution (O.3gjlOO幻11).After 2

the sections ('，re ¥ivashed with watεr and ob:弓erved.

Sections of the seta of are treated with 2 of green

solution AJter 10 these sョctionsare tr色atεdwith one of eosin

solution After 2 the sections are washed with water and obsenled.

Sections of the st日rnof i.'qa Y'l7:a附~a 戸 ιre treated with 4 of gaen

solution lHter 10 these s日ctionsare treatedwitr， one of eosin sobtion After 2 tbe sections are vvashed with vmter and observed.

SectiO':ls of the stem ofηωηρare treatedwith une of

grεen solutionAfter 30 these sections are treated wi十b of

eosIn soluti.on After 2 thεsect10ns are washed with 'i'Tater and observeι

3. 01tlserva'tilJlll and DlIsiCii!Ss.iun

3.1 R記lati⑪iilshipsbetween inn即日tructur・iESuf stem amll seta

1n ninεof th巴 stemis differentiated into A (epid町 B

C D (stereome) ancl E The seta Is di紅白rentiatedinto F-， K同ヲ

Gamdophytc in Ml1sci

L国， M-:lnd N Jano II)< both the stem anc1 seta of

司、

are

differ日ntiateclinto five tissues<

1n elevεn S7つeciesof Br ot two of Mniaceae and six

of the stem differentiated into A 月ndE

τhe seta is clifferentiated into }I¥L-M-and

both the stem and seta are diff台r日ntiatεdint心 fourtissues< This in.dicatio.n 01:

paralleJism in diff合I合ntiationin tI1e stenl and seta 1)リ

4

三f_~A : Epidermis， B : Cortex， C : Leptome、D:St日 eorne，E : Hydrome or Hadrome，ト・ Present

4 1. KAWAI

iL 2

cell waHs of the leaf are stained and cell

walls of the epidermis， rεd As well as， in the stem of cell vvalls of

the are stained red In the stεm of 幻unτノ cel1walis of the

outer-most a1'εoften stained and those of tl:lεnextinner rεd

(Plate V-3 and 4)， These tissues similox are considered

and tht2S the stem of

;mc1 Bartramia. And ;n the stem ofμ11ty tけ;

f 1n 即y伊'pl幻削tωzμ仰t幻仰仰?刀m肌?悦伐1丸， r町1児ea訂 a叶II口DO町r比吋t廿10仇r♂]βs0吋

the stem are

10 the seta of

stained

0:[ seta and in the outer-most

cell walls of the outer司mostεr(瓦)are

and ザ1，the outer-most

of st町 na了ecOl1s:derec1. 2. similar

chare:cteristic may exist between them，

:1， 3 ReiauilJJnship hehveel1l the f⑪rmaHon of st肥man!:i， s巴taRn Polytrklhml1L

In a cross section of the stea， ceH which cut off three has three

faces

Each is divided into fIv日 川10仁ellsfG: leaf in the oate了-n"，ost

cortica: initiaj) in the middiε 11 one ceι

di'Jided into A cell

and B ceI1

The CDE-cel日1of the ce1t廿ra剖IIpo印rρ寸t討ionis 凶I日laH匂ydivided i旧nt凶oC cel口: D-cεl日1(hydromヨlesh合矧臼a一じ巾h】干吋E寸i凶ni抗ti均aiリ)and E一C印色]日l(小山!r勺t叩 -1εe凶-1泊ni抗 The zygote

is dividεd into quarters (Plate VI-lb)， Each of which

of division of the sten丸 isdividεd ir;to fiγe tvvo cel1s

tvvo cells ロthemiddle 2nd one cell

KL-cell is periclinal1y divided into K-and L-cells

divided into 1¥1[ -and N悶cells

In the stem， the outer-;nost cε11 IS仁 intoa leaf. If diffεrentiation patterns

and similar are considerεd司 inJ'9olvtrichurn， the outer-DJOst

。fthe seta appears to have leaf-like

Refenmces

E. ¥N ater conduction in commαne， Ann. Bot. 45: 175四200目

Thεmechadsm of water conduction in the musci considered in relation to habit.

Ana Bot. 47: 401.422ラ 889巴912.

目立BANT，C， Structure et differentiation des tissus condlじcteursdans 112

des Polvtrichum. I'hturalia monsoelieasia. ser. BoL 18: 293-318

Ga日1etodwt己 inIv勺U-S仁1 5

their ，;tn;ctm色ydisi:ribution and 390-4lUo

C~hs合rvatÍüns sur les traces foJjョiresdes lYl0SSeS S. str.

chez des ε!(じコme三rltsa c♂a一ra臼ctのう勺 ~j r.:: 】().{L~: I ，J~i-' {;JO.

of i:he in the gam，~to

1. JVIisCE:llaneous notes on of

in histo】anatomiocal"tructures. J. Hattori BoL Lab. 3'7

211-227.

of ぉ ~:TuctíJrεc F elements in Livが~-v/orts and

mOSfoes. J. Hattori Bot. Lab. 37: 229-234。

一一 Studieson the devε!opm削 ofthe

。f80汀lePolv十richales.II. aT¥d structure ai c.entral ;..;'trand. ]. J-Iattor:l Iiot， l..ab. 38: 565..607.

iηthε

and J.円以仁oriBot. Lalコ 3~~Þ: 235ω254.

一一一 anatorl1y01 thεgametophy十esin Dai，vsonia

.J. Hatl:ori BoL La:) 40: 221246.

一一一 The tissue of J. Gramer Va仁川'7

1. 5t01CEes on :h2 広I:he

in the T¥1usci. (2) (Jn the seta in SOi11e

Kanazawa Univ. 1，1・39..57

tissue of the

of D8.w出仁川lIa

and K. IICEDi¥

in Musci. (1) On th杷 tissvεo{th色stem111 some

一一一一一

一 一 (J倒的 Die

thεin~]εr strllctures of the stem in some

am心nganatomical ch日iX;'::にterlsticsof sterns in， sorne

Kanazavva Univ. 24: 13-43.

ロnddes ZellteiIu日 inden (1)

studies on I:he. tissue of the in 1¥1ωci. (12)

AnatomicaI characteristics of stem in the sonle of Bartrarr，iaceaeo Sci

!(anazaωα 仁川iv.26: 31同56.

一一 S.YOSHITAKE and M. -'lAIV[AZAKI of the st臼.nthrough

in comrnuηe HEDiN. and

Ka仰 zawaUniv. 3ifJ: 47-53

一一，一 andE. YAlVIAlvIOTO of the stem of

M.niurn and Fissidens. Sci。 Ka!1ozaw♂ Univ. 31: 31-42

6 10 KAVIAI

and Ho OCHI bet"1I1een taxonomic and anatoγnical

Sci. ReiJ. J{，β幻α2'0ω Univ.32: characteristics of stems in some

1-67.

vv固 Ueberdie Haじstorialschlaucheam F国 sedε1伊 Laubmoose.Bero Deutsch Bot. Ges. L!3: 262-270。

P. G. Grundlinien zu einεvendeichenden Anatomie der Laubmoose.

WlSS. ヨot.G: 363-4660 C園 M. Las de Phi1onotis del sur de

Rev. Muso Cs. Nat. Bermardino Revadav必 Bot.3: 185-234

一一一 Elgenero Breutelia 討 en1a re;.rion Andino-oatagonica. Rev. Mus.

Cs.Nai. Bernωrdino Revadavia Bot. L1: 321-360

T. and A. NOGUCHI

flattori Boto Lab 0 ~27: 361目3650

PAR. anどIits allies. J

11斗nu

ワムM

114

ヴJt

-ー一日。。“41hu a

)

t

zdTY

PO

ー

サ

ゾ

十

L

Uιo

jxB

L Introduc!:ion and the genus j 0 1-i 2ttcri

11. The genus

• ]， I-!ailoTi .Bot， L.ab々29:1-52.

Alahabado

al1d London

Aηη.

Bot. 15: 1-38.

Conduction of ion solution and assimilate~; in

vnLLD. Biol. 719-727.

der Pflanzeno VII. Ueber den anatomischen Bau

des rv:J:oostammes. Sitzber. matho符atoG A初 Wiss.Wien. t!13: 497-5180 s. R On the anatomy and εnt the sDoroR:onium of th色 HlOsseso

L仇η固 80c.Bot， 24: 262-285

E. V. The structure and life of コ London

E. and TRACHTENBERGW at2r movernεnt throul!h hvdroids of a moss

Jsrael louro Boto 25: 168-173.

Plates

8 LKλ'iNAI

Plate I. Cross sections of the stems in Polytrにhacea巴 (KAWAIand IKEDA 1970) o.nd thεir seto.e (KAWA! 1969)ーA: EpIdermis， B: Cortex， C: Leptome， D: Ster色ome，E: Hydrome， 1a: Stem ofコ'olytrichumβilijerumI-!EDW， x80，

lb: Seta of Polytrichum pilife仰 m HEDW. xl00，

2a: Stem of Polytrichum commune HEDW. x80， 2b: Seta of Polytrichum commun巴 Hedw.

30.: Stem of Pog口nalumn~tう声onicum NOG. o'nd OSADA x120，

3b: Seta of Pogonatum niJう戸onicumNOG. and OSADA )[100， 40，: Stem of 1コ'olytrichumfuηかerz河umHEDW. x80， 4b: SetO， of Polytrichum funiperinum HEDW， xlOO，

5a: Stem of Atrichum undulatum (HEDW，) P. BEAUV， x80，

5b: Seta of .Alrichum unclulat;ム1m(HEDW，) P. 3EAUV， xlOO，

6a: Stem of Pogo日atui11gra削 lijolium(LINDB.) JAEG. x80，

6b: Seta of Pogo叩 tumgl叩 difolium(LINDB.) JAEG， x80

Gametophyte in Musci 9

Plate 1

10 1.KAWAI

Plate 11. Cross sections of th巴 sternsil1 ?olytrichaceae (KAWAI and IKEDA 1970) and

Bryaceae (KAWAI and OCI-lI 1987)， and theh占 setae.< Epid巴γmis，B: Cortex， C: Lεptomε， D: Stεreome， E: Hydrome (Hadrom号)

la: Stem of Pogonaturn contortu持zMENZ， x80，

lb: Seta of Pogonatum coηtortμm l¥l!ENZ， xlOO，

2a: Stem of Pogo河atuminflexum (L!NDB.) P AR x80，

2b・Setaof Pogo将atuminflexum (LINDB，) P AR x100，

30.: StεHl of Polytγichum formosum HEDW， xlOO，

3b: Sεta of Polytrichum formosu711 HEDV{， xlOO，

4a: Stem of Pohlia flexuosa HOOlC x110，

4b: Seta of Pohlia flexuosa I-IOOK. x200， 5a: Stem of Pohlia nutans (HEDv;r，) LINDB， xllO，

5b: Seta of Pohlia nuta苅S(HEDV¥T，) LINDB. x150，

6a: Stem of Acidodoniium exaltalum (M!TT，) JAEG， x100，

6b: Seta of A.cidodo河tiU'n1 exaltat-urn (IvIITT.) JAEG. x150.

qL

eg

qc

eャ

qg eg

ez ql el

11 al匂Id

n psnw u!訂正qdO:)8UI官。

12 l.KAiJiTAI

Plate m. Cross s日ctionsof the st日n1Sin Bryacea巴 (KAWAI and OCHI 1987) and their setae.A: Epidermis， '3: Cortex， C: Leptome， E: I-!adrom色

1a: Stenl of Bryum dichotomum HEDW. x140，

lb: Seta B小川 d川 otOlnumHEDW. x200， 231: Stem of Bryum soboめをrumTOYL. x110， 2b: Seta of Blyum sobo!iもrumTOYL. x150，

Stenl 8，f BryuJn turbinatum (I--IED'iV.) TURr.L xl10) 3b: Seta Bryum lurbinatuJ!l1 (I-IEuVl.) 'TURN-o x150，

431: Stem of Brachymenium lanceolatum HOOK. f. et iN!LS. x140， 4b: Seta of Brachymenium lanceolatμm HOOK. f. et VvILS. x200， 5a: Stem of BrachymeniuTYI le戸to戸hyllum(C. rVlUELL.) JAEG. xlL!O， 5b: Seta of Brachym.e持iumleptophyllum (C. IVJ:UELL.) JAEG. x200， 631: Stem of Brachymeniurn exile (Doz匂 etMOLK.) BOSCH. et LAC. x140， 6b: Seta of Brachy押-zeniu'Jnexile (Doz.巴tr，IIOLK.) BOSCH.巴tLAC. x2CO，

7a: Stem of Brachyme抑制~ pulchrum HOOK. x140，

7b: S巴taof Brachyrneniunl，かdchrumHOOK. x200，

8a: Stenl 01: Bra.chYl抑enzμ よ似合itulatum(M!TT.) K!NDB. x110， 8b: Seta of Brachymeηium capitulatum (MlTT.) ){INDB. x200.

Gametophyte in Musci 13

Plate 111

lb

3a

7a 8a

7b

14 LKAVv'AI

Plate IV. Cross sections of the stems in I/Iniacea思 (KAWAI1971)， Bartramiaceae

(KAi可Al1981) and Dicranaceae (KAWAI 1978). and their setae

A: Epidermis， B: Cori:ex， C: Leptome， E: Hadrome

1a: Stem of Mnium microthyllum Doz. et NIOLK. x80，

1b: Seta oI Mnium microphyllum Doz. et MOLK. x100，

2a: Stem of 111'ηium stバaiulumNUTT. x80， 2b: Seta of 11グniumstriatulum NIITT. x80，

3a: Stem of Philoηotis falcata IVIITT. x200，

3b: Seta of Philonotis faicala lVl!TT. x200， 4a: Stεm of Philo日otisthwaitesii NIITT. x200，

4b: Seta of Philonotis u以uaitesiiMlTT. )[150，

5a: Stern of .Di・cranumviride v. llakkode何se(CARD. ) T AK. x140，

5b: Seta of Dicranum viride v. ha絞っdense(CARD. ) T AK. x:200， 6a: St巴m of Dicァanumniptonense BESCI-!. x110，

6b: Seta of Dl:cranum刃物onenseBESCH. )(150，

7a: Stern .Oicranum japonicum MlTT. xllO， 'lb: Seta Dicranum japonicum l¥I!ITT. )[150，

8a: Stem of Dicnωlum scoTarium HEDW. x80，

8b: Seta of Dicranurn scotariurn HEDVv. x120，

9a: Stem of ~Dícranu1~同 bowringii ~'iiITT. x150，

9b: Seta of Dicra仰 m bowringii t¥!I!TT. x150，

10a: Stεm of Car紅白ytc戸odiurn似品thorocladium(c. MUELL.) BESCH. x140，

10b: Seta of Campylobodium euthoroιladium (C. !VIUELL.) BESCH. x200

5a ~ AdOE 説。?につ庁C!IB'G~ ofEO、なく〉J

。(今

Gametophyte in Musci 15

Plate IV

16 !.KAWAI

Plate V. Properties showing similar staining.

Fig. 1. Cross section of the stem in Polytηchum com開封ηeHEDW。1: Leaf (blue-gre巴n)，2: Epidermis (red)， 3: Cortex (blue-green)。

Fig. 2. Cross section of the stem in Bartramiaρomiformis HEDW. 1: Epidermis (red)， 2・Cortex(bluε-green)

Fig. 3. Cross section of the st巴min I-lypηumβlumaefoァmeWILS 1: Lear (blu巴血green)，2: Epidermis (red) ， 3: Cortex (blue-grεen)

Fig. 4. Longitudinal section of the stem in I-fytnum βlumaeン伽meiiITlLS 1: Leaf (blue-green)， 2: Epidermis (red) ， 3: Cortex (blue-green)

Fig. 5. Cross section of th巴 setain Polytrichum commuηe HEDW

1: Outer-most layer (blue-green)， 2: N ext in日巴rlayer (aゴ).

Fig. 6. Cγoss section of the seta in Pogonatum inflexum (LJNDB.) LAC

1: Out色r-most1乱yεr(blue-green)，と Nextinner layer (red).

Gametophyte in Musci 17

Plate V

18 I.KAWAI

Plate Vl. Relationships between th巴 earlyformation of stem and seta in Polytrichum

Fig. 1a. Apical cell with three merophytes.

Fig. 2a. Each merophyte dividing into two leaf-initials (G)， two cortical.initials (AB)

and a medullary-initial (CDE)

Fig. 3a. Merophyte is differentiated into leaf (G)， epidermis (A)， cortex (B)， and

medullary.initial (CDE)

Fig. 4a. Merophyte is differentiated into leaf (G)， epidermis (A)， cortex (B)， leptome

(C)， stereome (D) and hydrome (E)

Fig. 1b. Zygote is divided into four merophytes.

Fig. 2b. Each merophyte dividing into two cells (F) in outer.most portion， two cells

(KL) in middle portion and one cell in central portion (MN).

Fig. 3b. KL-cells are divided into K-cell and L-cell

Fig. 4b. Merophyte is differentiated into the outer-most layer (F)， next inner layer (K)。

Gametophyte in Musci 19

Plate VI

1a 1b

@

⑦ 2a 2b

3a 3b

4a G

4b