EBT GeneBoy NuriaAnon LACC2
of 4
Transcript of EBT GeneBoy NuriaAnon LACC2
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OPERACINS SINXELAS CON SECUENCIAS
Nuria An Liares LACC 2
Secuencia original:
ATGTGCGACCATACCAAGCTGAAAATACTGCATCCCGTCTGATCTGCACAGTCAAGCAGCTTAGGGCCC
AGTCAGTAGTGCGGTGGGGGACCATGCGCGAACATTGTGGTGTTGCACTTATGTGCGACCATACCAAG
CTGAAAATACTGCATCCCGTCTGATCTGCACAGTCAAGCAGCTTAGGGCCCAGTCAGTAGTGCGGTGG
GGGACCATGCGCGAACATTGTGGTGTTGCACTTATGTGCGACCATACCAAGCTGAAAATACTGCATCCC
GTCTGATCTGCACAGTCAAGCAGCTTAGGGCCCAGTCAGTAGTGCGGTGGGGGACCATGCGCGAACAT
TGTGGTGTTGCACTTATGTGCGACCATACCAAGCTGAAAATACTGCATCCCGTCTGATCTGCACAGTCA
AGCAGCTTAGGGCCCAGTCAGTAGTGCGGTGGGGGACCATGCGCGAACATTGTGGTGTTGCACTT
a) Secuencia reversa
TTCACGTTGTGGTGTTACAAGCGCGTACCAGGGGGTGGCGTGATGACTGACCCGGGATTCGACGAACT
GACACGTCTAGTCTGCCCTACGTCATAAAAGTCGAACCATACCAGCGTGTATTCACGTTGTGGTGTTAC
AAGCGCGTACCAG
GGGGTGGCGTGATGACTGACCCGGGATTCGACGAACTGACACGTCTAGTCTGCCCTACGTGATAAAAG
TCGAACCATACCAGCGTGTATTCACGTTGTGGTGTTACAAGCGCGTACCAGGGGGTGGCGTGATGACT
GACCCGGGATTCGACGAACTGACACGTCTAGTCTGCCCTACGTCATAAAAGTCGAACCATACCAGCGTG
TATTCACGTTGTGGTGTTACAAGCGCGTACCAGGGGGTGGCGTGATGACTGACCCGGGATTCGACGAA
CTGACACGTCTAGTCTGCCCTACGTCATAAAAGTCGAACCATACCAGCGTGTA
) Secuencia co!"le!en#aria$
TACACGCTGGTATGGTTCGACTTTTATGAC
GTAGGGCAGACTAGACGTGTCAGTTCGTCGAATCCCGGGTCAGTCATCACGCCACCCCCTGGTACGCG
CTTGTAACACCACAACGTGAATACACGCTGGTATGGTTCGACTTTTATGACGTAGGGCAGACTAGACGT
GTCAGTTCGTCGAATCCCGGGTCAGTCATCACGCCACCCCCTGGTACGCGCTTGTAACACCACAACGTG
AATACACGCTGGTATGGTTCGACTTTTATGACGTAGGGCAGACTAGACGTGTCAGTTCGTCGAATCCCG
GGTCAGTCATCACGCCACCCCCTGGTACGCGCTTGTAACACCACAACGTGAATACACGCTGGTATGGTT
CGACTTTTATGACGTAGGGCAGACTAGACGTGTCAGTTCGTCGAATCCCGGGTCAGTCATCACGCCACC
CCCTGGTACGCGCTTGTAACACCACAACGTGAA
c) %rascri#o &e ARN$
AUGUGCGACCAUACCAAGCUGAAAAUACUGCAUCCCGUCUGAUCUGCACAGUCAAGCAGCUUAGG
GCCCAGUCAGUAGUGCGGUGGGGGACCAUGCGCGAACAUUGUGGUGUUGCACUUAUGUGCGACC
AUACCAAGCUGAAAAUACUGCAUCCCGUCUGAUCUGCACAGUCAAGCAGCUUAGGGCCCAGUCAG
UAGUGCGGUGGGGGACCAUGCGCGAACAUUGUGGUGUUGCACUUAUGUGCGACCAUACCAAGCU
GAAAAUACUGCAUCCCGUCUGAUCUGCACAGUCAAGCAGCUUAGGGCCCAGUCAGUAGUGCGGU
GGGGGACCAUGCGCGAACAUUGUGGUGUUGCACUUAUGUGCGACCAUACCAAGCUGAAAAUACU
GCAUCCCGUCUGAUCUGCACAGUCAAGCAGCUUAGGGCCCAGUCAGUAGUGCGGUGGGGGACCAUGCGCGAACAUUGUGGUGUUGCACUU
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&) Pro#e'na co&i(ica&a es#ruc#ura "ri!aria)$*Can#as solucins "osiles +ai "ra a secuencia
"role!a, *Por-ue,
La transcripcin no siempre comenzar en el mismo punto. Podr comenzar por A,U o G demanera que podremos encontrarnos con tres lecturas diferentes:
-Lectura :
!C"#T$L$%L#P&'(A)(((LGP()'CGGGPCA*%&&L#LCAT%P('$+C%P("L#()AA'GP&((A
&G"#ATLCCT+&P+)A*TA(L%CT&$)LA)(&>!#CG&AL!C"#T$L$%L#
P&'(A)(((LGP()'CGGGPCA*%&&L#
-Lectura /:
CAT%P('$+C%P("L#()AA'GP&((A&G"#ATLCCT+&P+)A*TA(L%CT&$)LA)(&&
GT!#CG&AL!C"#T$L$%L#P&'(A)(((LGP()'CGGGPCA*%&&L#LCAT%P('$+C%P(
"L#()AA'GP&((A&G"#ATLCCT
-Lectura 0:
&P+)A*TA(L%CT&$)LA)(&>!#CG&AL!C"#T$L$%L#P&'(A)(((LGP()'
CGGGPCA*%&&L#LCAT%P('$+C%P("L#()AA'GP&((A&G"#ATLCCT+&P+)A*TA(
L%CT&$)LA)(&>!#CG&AL
e) Es#a&'s#ica &a secuencias
e$.)Porcenta1e de nucletidos.A: / nucletidos2 /0,3/4
C: /5 nucletidos2 /3,/4G: 06 nucletidos2 /7,384
T: 57 nucletidos2 //,674
e$2) Porcenta1e de dimeros 9pare1as de ases diferentes;
AA: /< dimeros2 3.5
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e$/)Porcenta1e de tripletes 9codones;
AAA: 7 tripletes .674
AAC: < tripletes 5.7
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GTC: / tripletes /.3/4
GTG: /5 tripletes
