Amish JSHS Poster Presentation Landscape

1
www.postersession.com Introduction Conclusions COAXIALLY ELECTROSPUN FIBROUS SCAFFOLDS CREATED BY A CONVENIENT, SELF-DEVELOPED ELECTROSPINNING APPARATUS Amish Patel Montville Township High School Acknowledgements Thanks to… Dr. George Collins and his summer research discussion group for their advice and guidance Jennifer Moy for teaching me lab procedures and assisting me in the lab as well as Sahitya Allam for volunteering to do my SEM imaging for me. Mr. John Hoinowski for helping me construct the apparatus Dr. Michael Jaffe and the NJIT Department of Biomedical Engineering for providing amazing research experiences over the past two years Ms. Salazar, Dr. Brinkman, the Science Research course, and my parents for their support over the past three years The SEM images taken show evidence of zein fibers, which proves that the apparatus constructed successfully produced coaxial fibers. However, it is reasonable to doubt that conclusion due to the abundance of a film in the image, which is likely PCL. This film prevented us from using ImageJ software to measure the fiber diameter because the software could not discern between the background and fiber. More refined methods can be used to amend this problem, including using a different organic solvent to dissolve PCL, increasing the time given to allow PCL to dissolve, or using an electrospinnable polymer that dissolves easily. The fact that zein fibers were successfully formed proves the viability of the apparatus used in this study for use in tissue engineering and drug delivery studies. The apparatus constructed can be modified such that the coaxial orientation is implemented on a ForceSpinning system to drastically increase the production rate of these fibers so they can be used clinically. This is an important step in allowing electrospinning biomaterials to be used in everyday life. Needle Several studies show the use of coaxial electrospinning, a technique in which a core polymer flows inside a sheath polymer, forming a concentric morphology. This formation allows one to significantly modify the properties of electrospun polymer materials and improve their efficacy in biomedical engineering applications. Abstract Experimental Design Sheath Polymer Solution Poly(ε-caprolactone) (PCL) in methylene chloride Core Polymer Solution Zein protein in 80% ethanol and 20% dH 2 O Needle Figure 1: Electrospinning a polymer within another polymer creates a concentric fiber morphology, seen in the cross-section on the right. Electrospinning has been used as a reliable method of creating polymer nanofibrous constructs for applications in fields such as tissue engineering and drug delivery. However, the use of a single polymer has limited the potential of these constructs. Coaxial electrospinning is a novel method that has prospects of increasing the versatility of electrospun materials. This version of electrospinning utilizes a coaxial fiber of concentric morphology with a core polymer within a sheath polymer. This coaxial fiber may be a better option in tissue engineering and drug delivery because it has extended capability as a result of using two different polymers. Studies have shown the use of this morphology in improving hydrophobicity, tensile strength, conductivity, and other crucial properties used in mimicking the environment of cells or delivering drugs. In this study, we design and construct a functional coaxial electrospinning apparatus to spin discernable fibers with a concentric, dual-phase morphology for tissue engineering and drug delivery applications. The apparatus is low cost and easy to operate. Figure 5: 1.50 mL/h Little evidence of fiber formation Small region in which fiber- like structures can be observed Figure 4: 1.25 mL/h Image shows the presence of fibers in some areas A film still covers most of the fibers All images show evidence of a film (likely to be PCL) obstructing the view of the fibers, yet they are still in a concentric morphology. Figure 6: 1.50 mL/h Images of coaxially electrospun materials after electrospinning Flow Rates Tested (mL/h) PCL Sheath 1.10 (Control) Zein Core 1.00 (Control) 1.25 1.50 Materials were electrospun using the above flow rates (3 total materials electrospun) PCL sheath dissolved afterwards for analysis of core to confirm formation of concentric morphology Quantitative Analysis Find fiber diameter for each sample using ImageJ software Qualitative Analysis Observing the presence of fibers in SEM images Sheath Polymer Solution Core Polymer Solution A: 1.00 mL/h B: 1.25 mL/h C: 1.50 mL/h Results Methods Results Figure 3: 1.00 mL/h Fiber-like structure can be seen throughout Considered evidence of fibers Electric Field Surface Tension High Voltage Power Source Ground Solution-loaded syringes Construction of Apparatus 2a Figure 2: a) Setup of apparatus during electrospinning. b) Core solution needle is inserted into sheath solution needle through a Luer male to female elbow adapter. Syringe with core solution Syringe with sheath solution Results Methods

Transcript of Amish JSHS Poster Presentation Landscape

Page 1: Amish JSHS Poster Presentation Landscape

www.postersession.com

Electrospinning is a technique used to fabricate polymer materials with exceptional properties that are useful in tissue engineering and drug delivery. These materials, however, are limited to the properties of the polymers used to make them, which has been a problem in recent years. Several studies show the use of coaxial electrospinning, a technique in which a core polymer flows inside a sheath polymer, forming a concentric morphology. This formation allows one to significantly modify the properties of electrospun polymer materials and improve their efficacy in biomedical engineering applications.

Introduction

Conclusions

COAXIALLY ELECTROSPUN FIBROUS SCAFFOLDS CREATED BY A

CONVENIENT, SELF-DEVELOPED ELECTROSPINNING APPARATUS Amish Patel

Montville Township High School

Acknowledgements

Thanks to…

Dr. George Collins and his summer research discussion

group for their advice and guidance

Jennifer Moy for teaching me lab procedures and assisting

me in the lab as well as Sahitya Allam for volunteering to

do my SEM imaging for me.

Mr. John Hoinowski for helping me construct the apparatus

Dr. Michael Jaffe and the NJIT Department of Biomedical

Engineering for providing amazing research experiences

over the past two years

Ms. Salazar, Dr. Brinkman, the Science Research course,

and my parents for their support over the past three years

The SEM images taken show evidence of zein fibers, which proves that the apparatus constructed successfully produced coaxial fibers. However, it is reasonable to doubt that conclusion due to the abundance of a film in the image, which is likely PCL. This film prevented us from using ImageJ software to measure the fiber diameter because the software could not discern between the background and fiber. More refined methods can be used to amend this problem, including using a different organic solvent to dissolve PCL, increasing the time given to allow PCL to dissolve, or using an electrospinnable polymer that dissolves easily. The fact that zein fibers were successfully formed proves the viability of the apparatus used in this study for use in tissue engineering and drug delivery studies. The apparatus constructed can be modified such that the coaxial orientation is implemented on a ForceSpinning system to drastically increase the production rate of these fibers so they can be used clinically. This is an important step in allowing electrospinning biomaterials to be used in everyday life.

Introduction

Needle

Several studies show the use of coaxial electrospinning, a technique in which a core polymer flows inside a sheath polymer, forming a concentric morphology. This formation allows one to significantly modify the properties of electrospun polymer materials and improve their efficacy in biomedical engineering applications.

Abstract

Experimental Design Sheath Polymer Solution – Poly(ε-caprolactone) (PCL) in

methylene chloride

Core Polymer Solution – Zein protein in 80% ethanol and

20% dH2O

Needle

Figure 1: Electrospinning a polymer within another polymer creates

a concentric fiber morphology, seen in the cross-section on the right.

Electrospinning has been used as a reliable method of creating polymer nanofibrous constructs for applications in fields such as tissue engineering and drug delivery. However, the use of a single polymer has limited the potential of these constructs. Coaxial electrospinning is a novel method that has prospects of increasing the versatility of electrospun materials. This version of electrospinning utilizes a coaxial fiber of concentric morphology with a core polymer within a sheath polymer. This coaxial fiber may be a better option in tissue engineering and drug delivery because it has extended capability as a result of using two different polymers. Studies have shown the use of this morphology in improving hydrophobicity, tensile strength, conductivity, and other crucial properties used in mimicking the environment of cells or delivering drugs. In this study, we design and construct a functional coaxial electrospinning apparatus to spin discernable fibers with a concentric, dual-phase morphology for tissue engineering and drug delivery applications. The apparatus is low cost and easy to operate.

Figure 5: 1.50 mL/h

• Little evidence of fiber

formation

• Small region in which fiber-

like structures can be observed

Figure 4: 1.25 mL/h

• Image shows the presence

of fibers in some areas

• A film still covers most of

the fibers

All images show evidence of a film (likely to be PCL) obstructing the

view of the fibers, yet they are still in a concentric morphology.

Figure 6: 1.50 mL/h Images of coaxially electrospun

materials after electrospinning

Flow Rates Tested (mL/h)

PCL Sheath 1.10 (Control)

Zein Core 1.00 (Control) 1.25 1.50

• Materials were electrospun using the above flow rates

(3 total materials electrospun)

• PCL sheath dissolved afterwards for analysis of core to

confirm formation of concentric morphology

• Quantitative Analysis – Find fiber diameter for each

sample using ImageJ software

• Qualitative Analysis – Observing the presence of

fibers in SEM images

Sheath Polymer Solution

Core Polymer Solution

A: 1.00 mL/h B: 1.25 mL/h C: 1.50 mL/h

Results Methods Results

Figure 3: 1.00 mL/h

• Fiber-like structure can be

seen throughout

• Considered evidence of

fibers

Electric Field Surface

Tension

High Voltage

Power Source

Ground

Solution-loaded

syringes

Construction of Apparatus

2a

Figure 2: a) Setup of apparatus

during electrospinning. b) Core

solution needle is inserted into

sheath solution needle through

a Luer male to female elbow

adapter.

Syringe with core

solution

Syringe with

sheath solution

Results

Methods